Orris

1)Elaborate the electrophoresis technique used for the DNA isolation and analysis? 2) elaborate the electrophoresis technique used for protein isolation 3) what is native gel electrophoresis elaborate about the principles of isoelectric focusing electrophoresis 4) describe in detail a body working principle of a pH metre along with its application 5) what is electromagnetic spectrum describe its profit and in role in absorption spectroscopy 6)describe the working principle and application of HPLC 7) which chromatography technique used to isolation biomas on the basis of different charges and molecular weight and size 8) what is a principle of TLC mention about application of TLC

Reading File
This covers eight laboratory and analytical techniques:
  1. DNA electrophoresis: agarose gel electrophoresis, its principle, apparatus, procedure, staining, and interpretation.
  2. Protein electrophoresis: mainly SDS-PAGE, including protein separation, molecular-weight estimation, and protein isolation.
  3. Native PAGE and isoelectric focusing (IEF): separation of intact proteins by charge, size, shape, and isoelectric point (pI).
  4. pH meter: construction, working principle based on glass and reference electrodes, calibration, and uses.
  5. Electromagnetic spectrum and absorption spectroscopy: regions of the spectrum, interaction of radiation with matter, Beer-Lambert law, and analytical uses.
  6. HPLC: principle, components, working, common modes, detectors, and applications.
  7. Chromatographic separation of biomolecules: ion-exchange chromatography for charge; size-exclusion or gel-filtration chromatography for molecular size and molecular weight.
  8. Thin-layer chromatography (TLC): principle, Rf value, procedure, and applications.

Brief overview

These methods separate or measure molecules according to physical and chemical properties. Electrophoresis uses an electric field to move charged molecules through a supporting gel. Chromatography separates molecules through different interactions between a stationary phase and a mobile phase. Spectroscopy measures how molecules absorb electromagnetic radiation, whereas a pH meter measures hydrogen-ion activity in a solution.
Which topic would you like in detailed exam-note format first?
This is a shared conversation. Sign in to Orris to start your own chat.