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Transcription DNA to RNA
Presentation · PPTX
Here's what's inside the 23-slide deck (navy/teal-green color theme, designed for graduate/medical students):
| # | Slide |
|---|---|
| 1 | Title slide |
| 2 | Lecture outline |
| Section 1 | Central Dogma & Overview |
| 3 | Section divider |
| 4 | Central Dogma of Molecular Biology |
| 5 | Types of RNA (mRNA, tRNA, rRNA, snRNA, miRNA, lncRNA) |
| Section 2 | RNA Polymerases |
| 6 | Section divider |
| 7 | Table: Prokaryotic vs Eukaryotic RNA polymerases + inhibitors |
| 8 | Bacterial RNA Pol structure & σ-factor |
| Section 3 | Promoters & Regulatory Elements |
| 9 | Section divider |
| 10 | Eukaryotic promoter elements (TATA box, Inr, CAAT, DPE, enhancers) |
| 11 | General transcription factors (TFIIA–TFIIH) |
| Section 4 | Stages of Transcription |
| 12 | Section divider |
| 13 | Initiation → Elongation → Termination (detailed) |
| 14 | Two-column: Prokaryotes vs Eukaryotes comparison |
| Section 5 | RNA Processing |
| 15 | Section divider |
| 16 | 5' capping, 3' polyadenylation, splicing, alternative splicing |
| Section 6 | Regulation |
| 17 | Section divider |
| 18 | Prokaryotic operon model (lac/trp, repressors, CAP-cAMP) |
| 19 | Eukaryotic regulation (chromatin remodeling, HAT/HDAC, methylation) |
| Section 7 | Clinical Correlates |
| 20 | Section divider |
| 21 | Table: Rifampin, α-amanitin, actinomycin D, doxorubicin |
| 22 | Transcription defects: β-thalassemia, SLE, Fragile X, cancer |
| 23 | Summary slide |
Content sourced from Basic Medical Biochemistry 6e, Thompson & Thompson Genetics 9e, Emery's Medical Genetics, and Harper's Illustrated Biochemistry 32e.
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Transcription Study Guide
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Here's what's inside the multi-page A4 study guide (navy/teal color scheme, matching the earlier slides):
| Section | Content |
|---|---|
| Cover Page | Full-design cover with topics list and source citations |
| 1. Central Dogma | Overview, template vs coding strand, types of RNA table |
| 2. RNA Polymerases | Bacterial structure + sigma factors; Eukaryotic Pol I/II/III table with alpha-amanitin callout |
| 3. Promoters | Bacterial -10/-35 elements; Eukaryotic core promoter table (TATA, Inr, DPE, CAAT, GC box, BRE); enhancers/silencers |
| 4. General TFs | TFIID through TFIIH; PIC assembly cycle callout box |
| 5. Stages | Initiation, Elongation, Termination (prokaryotic Rho-independent/dependent + eukaryotic torpedo model) |
| 6. Comparison Table | 9-row side-by-side prokaryotic vs eukaryotic |
| 7. RNA Processing | 5' capping, 3' polyadenylation, splicing mechanism (lariat), alternative splicing; SLE/snRNP callout |
| 8. Regulation | Operon model table; eukaryotic TFs; chromatin remodelling; HAT/HDAC/DNA methylation; Fragile X callout |
| 9. Inhibitors | 5-drug table: Rifampin, alpha-amanitin, actinomycin D, doxorubicin, azacitidine |
| 10. Clinical Correlates | beta-Thalassemia, SLE, cancer (MYC/p53), mushroom poisoning |
| 11. High-Yield + MCQs | 12 high-yield flashcard facts + 5 exam-style questions with full answers |
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