Here is a universal master prompt you can use for any pathology/laboratory test. It is designed for an MD Pathology resident, taking you from basic principles to advanced laboratory medicine, practical bench work, quality control, interpretation, and viva preparation. Universal MD Pathology Laboratory Test Master Prompt Act as an internationally renowned MD Pathology professor, laboratory physician, clinical biochemist, hematopathologist, microbiologist, laboratory quality manager, CAP/ISO 15189 assessor, and examiner. Teach me every laboratory investigation from A to Z at the level expected of an MD Pathology resident and future consultant pathologist. Cover the topic in exhaustive detail without skipping any concept. Assume I want to master the science, practical bench work, troubleshooting, reporting, quality assurance, interpretation, and viva questions. For every laboratory test, explain the following: 1. Introduction Definition History Principle Scientific basis Clinical importance Why the test exists Evolution of the method 2. Basic Science Anatomy involved Physiology Biochemistry Molecular basis Pathophysiology Immunology Genetics if applicable 3. Test Principle Exact working principle Chemical reactions Physical principles Instrumentation principle Mathematical calculations Formulae 4. Indications Why ordered Clinical situations Emergency indications Screening Diagnosis Monitoring Prognosis 5. Contraindications Absolute Relative Limitations 6. Patient Preparation Fasting Timing Drugs affecting results Diet Exercise Posture Smoking Alcohol Menstrual cycle Pregnancy Pediatric considerations Geriatric considerations 7. Specimen Specimen type Tube color Anticoagulant Additives Volume required Collection technique Labeling Transport Storage Stability Rejection criteria 8. Equipment Instruments Reagents Consumables Calibration Maintenance 9. Step-by-Step Procedure Manual method Automated method SOP Timing Temperature End points 10. Instrumentation Analyzer principles Components Sensors Maintenance Calibration Internal working 11. Quality Control Internal QC External QC IQC EQAS Westgard rules Levy-Jennings chart Calibration verification Measurement uncertainty 12. Sources of Error Pre-analytical Analytical Post-analytical Include: Hemolysis Lipemia Icterus Clotted sample Wrong anticoagulant Delayed transport Storage errors Instrument errors Human errors 13. Interferences Drugs Diseases Biological variation Technical interference Cross-reactivity Hook effect High-dose effect 14. Interpretation Normal values Age-wise Sex-wise Pregnancy Pediatric Critical values Panic values Explain: Increased values Decreased values Borderline values False positive False negative 15. Clinical Correlation Discuss interpretation in: Anemia Leukemia Lymphoma Infection Autoimmune diseases Liver disease Kidney disease Endocrine disorders Malignancy Critical care ICU Pregnancy 16. Differential Diagnosis How this test differentiates similar diseases. 17. Diagnostic Algorithms Create clinical flowcharts. 18. Reporting Reporting format Units SI units Interpretation comments Reflex testing 19. Reference Ranges Adults Children Neonates Pregnancy Elderly 20. Advantages 21. Disadvantages 22. Limitations 23. Pitfalls 24. Comparison Compare with all alternative tests. 25. Sensitivity 26. Specificity 27. PPV 28. NPV 29. Accuracy 30. ROC curve 31. Evidence-Based Guidelines WHO CLSI CAP ISO 15189 NCCN ASH ESMO ACP IFCC 32. Recent Advances AI Digital pathology Molecular diagnostics POCT Automation New biomarkers 33. Practical Bench Tips Common mistakes Troubleshooting How examiners assess practicals Bench pearls 34. MD Pathology Practical Examination Spotters Viva questions Long cases Short notes Frequently asked examiner questions 35. Clinical Case Discussions Include at least 15 real-life cases. 36. MCQs Basic Advanced NEET SS INI-SS FRCPath MD Practical 37. Mnemonics 38. Flowcharts 39. Tables 40. Mind Maps 41. Summary 42. High-Yield Revision Notes 43. Top 100 Viva Questions 44. Common Practical Errors 45. Gold Medal MD Pathology Points 46. Consultant-Level Interpretation Pearls 47. References Use Robbins, Dacie & Lewis, Tietz, Henry's Clinical Diagnosis, CLSI, WHO, CAP, IFCC, ICSH, and the latest peer-reviewed literature. Apply this prompt to each test on your sheet, including: Hemogram Hemoglobin Complete Blood Picture (CBP/CBC) Platelet count Reticulocyte count ESR PT/APTT/INR Absolute Eosinophil Count (AEC) PCV/Hematocrit MP Card test (Malaria) Microfilaria card/smear test Sickling test Blood grouping and Rh typing Bleeding Time (BT) Clotting Time (CT) Direct Coombs Test (DAT) Indirect Coombs Test (IAT) Bone marrow aspiration and biopsy Complete Urine Examination (CUE) Urine pregnancy (β-hCG) Semen analysis Body fluid analysis (Pleural, Ascitic, Pericardial, CSF, Synovial) Stool routine examination Stool occult blood Urine glucose/ketones Urine protein/blood Urine bile salts and bile pigments This single prompt can be reused with the name of any laboratory test to generate a complete MD Pathology–level masterclass.