Can you prepare me for micro viva

Sl No. 1. 2. 3. 4. 5. 6. 7. Topic Practical arrangement list Role of Microbiology in diagnostic laboratory & Microscopy Robert Koch, Louis Pasteur (Images) Morphology & Physiology of Bacteria (including staining- gram stain and others); Overview of bacterial infection & pathogenesis; identification of bacteria-conventional, automated and molecular; AST- methods and interpretations; SVL Cell wall structure Bacterial growth curve Organisms causing infections based on route of transmission. Gram stain as slides • GPC (Staph/ Strepto) • GNC • GPB 9Corynebacterium, clostridium) • GNB AST methods Any PCR kit box and steps for PCR Sample collection & transport + DOAP, Culture media and methods (conventional & automated) + DAOP Sterile swab, VTM, Syringe with needle, Cotton, Gloves, RCM, Povidone iodine, Wilson Blair, Cary Blair, VR Medium, Universal container, Falcon tube, Vials for blood collection - Red, Yellow, EDTA, Blood culture bottle (Biphasic bottle), BACTEC bottle, Plain glass bulb (for CSF collection), ZN stain with slides, Albert stain & slides Blood agar, MacConkey agar, Nutrient agar & broth, Chocolate agar, TCBS agar, Loeffler serum, LJ media, DCA & XLD, Pot. tellurite agar, CLED, McIntosh & Fildes, Indole, MR, VP, Citrate, Urease, TSI for all ⊕ & ⊖, Catalase and Coagulase test ⊕ & ⊖, Oxidase ⊕ & ⊖, AST plate Antigen–antibody reactions (serological) & molecular methods of diagnosis : SVL ELISA plate, HIV rapid kit, HCV rapid kit, HbsAg rapid kit, Dengue rapid kit, Tridot CRP +ve & -ve ASO RF test Widal Test with tubes Malaria rapid kit RPR and TPHA General Virology & lab diagnosis of viral inf Tissue culture flask, Embryonated egg — diagram for egg inoculation, HIV Virus — structure (image), Herpes Virus — structure (image), Hepatitis Virus- structure (image) General Mycology & lab diagnosis Uninoculated SDA Candida – SDA Aspergillus – SDA Rhizopus – SDA Dermatophyte on SDA LPCB — Aspergillus, Mucor, Rhizopus, Dermatophyte CHROM agar with candida Penicillium – SDA India ink Slides — Aspergillus, Penicillium, BYC Germ tube test Parasitic infections & lab diagnosis Saline & Iodine wet mount Photos - life cycle, eggs —E. histolytica, Trichuris trichiura, Ascaris, Hookworm, Strongyloides, Giardia, Taenia spp. Infection control basics; 1. Uninoculated culture medias 8. 9. 10. 11. 12. 13. 14. Standard precautions, hand hygiene, PPE; AETCOM; 2. Transmission based precautions and HAI-VAP, CAUTI, CLABSI Sterilization BMW Bags, segregation chart Vaccines Hand wash, hand rub, scrub steps and materials used PPE kit Alcohol based hand rub bottle BMW bags — red, yellow, white & blue container N95 Surgical mask Chlorhexidine bottle Immunoprophylaxis, vaccines, immunotherapy BCG Vaccine DPT vaccine OPV Vaccine IPV vaccine Rotavirus HPV Vaccine Hep B Vaccine MMR Vaccine Pentavalent vaccine Rotavirus vaccine Tetanus toxoid Rabies-pre and post exposure schedule Vaccine Vial Monitor Overview of Bloodstream & CVS infections: RF, IE and methods of blood culture Microorganisms causing BSI. BHI broth, Castaneda agar, BACTEC Bottle, Streptococcus slide, ASO test, Dukes and Jones criteria, SOFA score for sepsis, Staphylococcus Slide, HACEK Case on IE, sepsis and RA Infections causing anemia infections Parasitic blood stream infections-part 1 (Malaria) Slide - P. falciparum and P. vivax ring and gametocyte form, life cycle of malaria, complications caused by P. falciparum, Malarial vaccine Case on malaria Parasitic blood stream infections-part2 (Kala azar, Filariasis) Image of LD bodies and microfilaria Case on Leishmania and filariasis lab diagnosis of HIV 1) Comb aids 2) Lateral flow assay 3) immune concentration Kits to be kept which are already tested Print out 1) Strategies of HIV testing 2) Exposure and source codes of PEP 3)HIV consent form and report format Case on HIV, cryptosporidium, TB (Opportunistic infections) lab diagnosis of enteric fever Lab D of enteric fever WIDAL – slide WIDAL – tube. Blood culture bottle BACTEC bottle 15. 16. Mac Conkey & NLF Wilson & Blair DCA XLD } Black colonies IMViC, TSI of S. typhi Motility Case on Typhoid fever lab diagnosis of brucella, leptospirosis, dengue fever, scrub typhus, candidemia Dengue Dengue RCT MAC ELISA (box) Rapid combo card test Truenat Box Scrub Typhus ELISA Box Agglutination box Scrub typhus- Weil Felix kit and ELISA kit insert Table of rickettsial infections showing species and disease caused. Tropical fever panel RT PCR kit insert and table showing which organisms are detected by it Leptospira- Rapid, ELISA Candida • Germ tube, • CHROM agar, • Dalmau plate • Candida - clinical image -onychomycosis, diaper candidiasis, intertrigo • Macroscopic-SDA with growth • Microscopy - BYC with pseudo-hyphae Case on Dengue, Leptospira, scrub typhus, Brucella and Candida lab diagnosis of bacillary dysentery : shigella, E. coli and others Enumerate Mos causing dysentery E. coli Mac – LF moist BA – grey β hemolytic GNR short rod slide I M Vi C U + + − − - TSI-A/A + gas Motility (+) Shigella Mac – NLF GNR – short rod slide I M Vi C U +/− + − − − TSI-K/A without gas & H₂S DCA – colourless 17. 18. 19. XLD – red colour Selenite F & tetrathionate broth Case on Shigella dysentry lab diagnosis of diarrhea: vibrio cholera and others Enumerate Mos causing diarrhoea 1) Gardner Venkatraman Classification 2) Colour printouts of blood agar and TCBS growth and gram stain of Vibrio cholerae 3) list of organisms causing diarrhea and dysentery Vibrio Universal container Cary Blair Alkaline peptone water TCBS – plain & c̄ yellow colonies BA c̄ hemodigestion. Mac c̄ NLF colonies (LLF if available) I M Vi C U + + – – – TSI-A/A without gas & H₂S. String test (prepare on time) Case on Vibrio lab diagnosis of amoebic dysentery and intestinal protozoan infection; stool examination •Stool examination: -Iodine mount -Saline mount •Focussed slide/photographs of: -Cyst and trophozoite of E.histolytica -Cyst and trophozoite of G.lamblia -Cyst and trophozoite of Balantidium coli •Life cycle of: -E.histolytica -G.lamblia Difference between bacillary and amoebic dysentery Case on Entamoeba lab diagnosis intestinal helminthic infection; stool examination Classification of helminthics and characteristics of cestode, trematode and nematode •Stool examination: -Iodine mount -Saline mount •Focused slide/photographs of: -Scolex of T. saginata -Adult worm specimen of Taenia species -Egg of H. nana (non bile stained) -Eggs of Schistosoma species showing -Egg of Fasciolopsis buski (operculated and bile stained) -Egg of Trichuris trichiura 20. 21. -Adult worm specimen of Trichuris (if available) -Planoconvex eggs of E. vermicularis -Adult worm specimen of E. vermicularis (if available) -NIH swab -Cellophane tape -Egg of Ascaris lumbricoides (fertilized, unfertilized and decorticated) -Adult worm specimen of Ascaris -egg of Hookworm (with 4 blastomeres /many blastomeres) -National Deworming Days •Life cycle of: -Taenia saginata -Taenia solium -Schistosoma -Fasciolopsis buski -Trichuris trichiura -E. vermicularis -Ascaris lumbricoides -Hookworm -Strongyloides stercoralis Case on Ascaris, neurocysticercosis Lab diagnosis of hepatic viral and parasitic infections Enumerate microorganisms causing hepatic viral and parasitic infections Hepatic Viral & parasitic infection HBs Ag – Rapid (+) & (−) HBsAg – ELISA box HCV – Rapid HCV Ab – ELISA box HAV, HEV – Rapid tests Graph for lab diagnosis of HBsAg HBV vaccine schedule & dose Entamoeba cyst & trophozoite Entamoeba life cycle Enterobius egg Enterobius life cycle & photo of egg Echinococcus life cycle. Fasciola life cycle & photo of egg Ascaris life & eggs photo. → Specimen – hydatid cyst & Ascaris Case on hepatitis A, B, E and C Lab diagnosis upper respiratory tract infections * Culture plates uninoculated and with growth of -S. pyogenes - Blood agar -S. pneumoniae - Blood agar and Chocolate agar -S. aureus - Blood agar and Nutrient agar -C. diphtheriae - Chocolate agar and Tm medium and Loeffler’s serum slope * Gram stained slides of -S. pyogenes -S. pneumoniae 22. 23. 24. 25. ZN stain and Lab diagnosis of TB pneumoniae and others, Gram Lab diagnosis of LRTI- S. stain revision Lab diagnosis of meningitis and encephalitis Lab diagnosis of UTI -S. aureus * Gram stain and albert stain slide of C. diphtheriae * GNB and GPC slides for students to perform Gram Staining * Nasopharyngeal swab * Sputum container * Chart for sputum grading Case on diphtheria, Streptococcus 1. Smear slides for ZN Staining 2. Steps of ZN staining 3. Wide mouthed screw capped container (for sputum collection) 4. Falcon tube 5. RNTCP guidelines for grading of ZN stained sputum smears 6. LJ medium 7. MGIT container 8. CBNAAT cartridge (old) 9. TRUNAT chip (old) 10. National TB day (24th March) - Theme for this year 11. NTEP treatment guidelines charts for DSTB, RRTB, MDR TB, XDR TB (Table) 12. 3+ stained smear 13. Modifications of ZN staining (different concentrations of H2SO4) Treatment BCG vaccine Case on pulmonary and EPTB BA uninoculated BA with alpha hemolysis, Gram staining photo, CRP test Neisseria Meningitidis photo of gram stain PCV vaccine Case on S. pneumoniae and N. meningitidis Microbes causing CNS infections CSF analysis table Neisseria meningitidis-gram stain GNB and GPC Negri body rabies Rhabdovirus photo Toxoplasma life cycle Cysticercus image from book with Lifecycle Cryptococcus slant and india ink photo with reagents Urease slant for cryptococcaus, SDA, chocolate agar Mucor SDA AND LPCB Tetanus and rabies classification Schedule of vaccination for OPV and IPV with dose Case of Neurocysticercosis, N. meningitidis, Rabies, Cryptococcus, Mucor Microbes Causing UTI 26. 27. 28. Lab diagnosis of genitourinary and sexually transmitted diseases Lab diagnosis bacterial skin infection Lab diagnosis skin infection- leprosy, fungal, viral Case on UTI- E.coli,enterococcus E.coli Mac – LF moist BA – grey β hemolytic GNR short rod slide I M Vi C U + + − − - TSI-A/A + gas Motility (+) Enterococcus Enterococcus - BA with no hemolysis, mac- pinpoint colonies, gram stain photo and bile esculin Enterococcus diagnosis flowchart Wet mount image- urine with pus cells and bacteria Schistosoma-life cycle, egg CLED Agar-inoculated and uninoculated Photos-microscopy and plating STD chart and Microbes N. gonorrhea – gram stain, chocolate agar Ulcer types - Table Tzank smear - herpes Syphilis - RPR, TPHA, VDRL TILE Case on Syphilis, N. gonorrhoea S. pyogenes-gram stain-GPC in chains +BA S. aureus- gram stain-GPC in clusters +BA Case - S. pyogenes, S. aureus, Cl. perfringens (Gas gangrene), Anthrax Ames transport medium Coagulase test - slide, tube CAMP test MRSA - AST plate Catalase ASO, CRP RCM - saccharolytic, neomycin BA, McIntosh Jar, Plain RCM, GPB with spores & direct smear B. anthracis - gram stain photo, medusa head appearance, bamboo stick Leprosy Ridley-Jopling classification - Table Clinical presentation images - nodular lesions, hypopigmented lesions, saddle nose, bony deformity AF smear of M. leprae NLEP notes - Emblem, Elimination date, Eradication program, World leprosy day Case on leprosy (Practical book-Pg224) FUNGAL Superficial Mycoses Tinea versicolor • Image-hypopigmented patch, Malassezia furfur Tinea Nigra • Clinical Image Dermatophytes • Uninoculated SDA • Case-Pg236 • Table-classification based on habitat • Clinical image-ring worm • Macroscopic, Microscopic and schematic diagram of: T.mentagrophytes, T. rubrum, M.canis, E. flocossum Subcutaneous Mycoses Mycetoma • Table – Agents • Clinical image • Clinical Case - Practical Book - Pg 239 Sporothrix - Microscopic image Chromoblastomycosis - Microscopic - Medlar bodies Rhinosporidiosis - sporangia with endospore image Opportunistic Mycoses Candida • Germ tube, • CHROM agar, • Dalmau • Candida - clinical image - onychomycosis, diaper candidiasis, intertrigo • Macroscopic - SDA with growth • Microscopy - BYC with pseudo-hyphae Penicillium marneffei (spp) -Microscopic image VIRUS HSV • Table - difference b/w HSV 1 & 2 • Clinical image - vesicular lesion on lip, Herpetic whitlow • Tzanck smear - multinucleated giant cell • Case (Pg 228) VZV • Clinical image - chicken pox & herpes zoster - segmented rash Human papilloma virus - image - warts Small pox - clinical image, Table - diff b/w chicken pox & small pox Measles • CPE - multinucleated giant cell - Warthin finkeldey cell • Vaccine Schedule Rubella • Clinical image - rash , cataract 29. Environmental surveillance, HAI, BMW management • Vaccine & National Immunization schedule BMW bags and boxes, hand hygiene, PPE Microbes causing HAI Water and air surveillance

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