1)Elaborate the electrophoresis technique used for the DNA isolation and analysis? 2) elaborate the electrophoresis technique used for protein isolation 3) what is native gel electrophoresis elaborate about the principles of isoelectric focusing electrophoresis 4) describe in detail a body working principle of a pH metre along with its application 5) what is electromagnetic spectrum describe its profit and in role in absorption spectroscopy 6)describe the working principle and application of HPLC 7) which chromatography technique used to isolation biomas on the basis of different charges and molecular weight and size 8) what is a principle of TLC mention about application of TLC Give 8 question give 8 marks answers
- DNA electrophoresis: mainly agarose gel electrophoresis for DNA separation, isolation, and analysis.
- Protein electrophoresis: mainly SDS-PAGE and related methods.
- Native PAGE and isoelectric focusing: separation of intact proteins by charge, size, shape, or isoelectric point.
- pH meter: glass-electrode principle, calibration, operation, and applications.
- Electromagnetic spectrum and absorption spectroscopy: interaction of UV-visible/IR radiation with matter for quantitative analysis.
- HPLC: high-pressure liquid separation based on differential interaction with stationary and mobile phases.
- Biomolecule isolation by charge, molecular weight, or size: ion-exchange chromatography, gel-filtration chromatography, and related methods.
- TLC: separation on a coated plate by differential adsorption/partition, with applications in identification and purity testing.
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