This composite of clinical photographs and diagnostic images details the workflow for Platelet-Rich Fibrin (PRF) preparation and its application in periodontology. The process begins with blood collection equipment, including a green tourniquet, butterfly needle with tubing, and a vacuum collection tube with an orange cap. Post-centrifugation, the blood tube demonstrates distinct layering: an upper clear yellow plasma layer, a middle PRF clot, and a bottom red blood cell mass. A technician is shown using sterile tweezers to extract the yellowish fibrin clot. The central panel illustrates processed PRF membranes resting on a perforated stainless steel PRF box used for compression. The final clinical photograph depicts the intraoral application, where a dental probe is used to place the PRF clot into a 4-6 mm periodontal pocket in a patient with periodontitis. The image demonstrates the transition from biological sample collection to a regenerative surgical adjunct for periodontal ligament and bone healing.

A multi-panel series illustrating the preparation and clinical application of Platelet-Rich Fibrin (PRF) in oral surgery. (A) Shows a clinical centrifuge used for blood processing. (B) Demonstrates a post-centrifugation glass test tube showing the separation of blood into three layers: a bottom layer of red blood cells, a middle 'buffy coat' PRF clot, and an upper layer of acellular plasma. (C) Displays the harvested PRF clot, appearing as a dense, yellowish-white fibrous gel. (D) An intraoral clinical photograph showing the PRF clot being placed into a fresh mandibular molar extraction socket to promote hemostasis and bone regeneration. (E) An intraoral follow-up photograph at the 7th postoperative day, showing the surgical site with blue monofilament sutures in place. The wound demonstrates primary intention healing with healthy pink gingival margins and reduced inflammation compared to the immediate postoperative state. This sequence illustrates the autologous regenerative process used to enhance soft and hard tissue healing in dental procedures.

This composite intraoperative clinical photograph illustrates the preparation and application of Advanced Platelet-Rich Fibrin (A-PRF) in oral surgery. (A) Extraction of an A-PRF clot from a centrifugation tube using forceps, showing the distinctive separation between the translucent yellowish fibrin layer and the dark reddish-brown erythrocyte-rich base. (B) Two harvested A-PRF clots placed on a sterile metal tray; the clots exhibit a gelatinous, yellowish texture with a concentrated red corpuscle portion at one pole. (C) Preparation of A-PRF 'corks' within a dedicated PRF box, where the material is compressed into cylindrical shapes for easier socket insertion. (D) Intraoperative view of the A-PRF material being applied into a mandibular extraction socket. The reddish-brown fibrin material is seen packed into the surgical site adjacent to existing molars to promote wound healing and bone regeneration. The sequence demonstrates the autologous blood processing technique used to enhance postoperative recovery and reduce complications like dry socket or excessive pain in dental surgery.