Here is the complete PPT content for your seminar on HIV Testing & Diagnosis, sourced directly from Park's Textbook of Preventive and Social Medicine.
SLIDE DECK: HIV Testing & Diagnosis
SLIDE 1 - Title Slide
HIV Testing & Diagnosis
- HIV Testing Strategies
- ICTC (Integrated Counselling and Testing Centre)
- HIV Counselling (Pre- and Post-Test)
- Diagnostic Tests
- HIV Screening in Special Populations
Source: Park's Textbook of Preventive & Social Medicine
SLIDE 2 - HIV Testing: Overview & Purpose
Why HIV Testing Matters
- HIV counselling and testing services in India run since 1997 under the National AIDS Control Programme (NACP)
- Goal: Identify people living with HIV as early as possible and link them to prevention, care and treatment
- Introduction of ART services in 2004 gave a major boost to testing programmes
- The "Test and Treat" policy aims toward UNAIDS fast-track targets (90-90-90 by 2020):
- 90% of PLHIV know their status
- 90% of those are on ART
- 90% of those have viral suppression
SLIDE 3 - HIV Testing Strategies
Two Main Approaches
1. Voluntary Counselling and Testing (VCT) / Client-Initiated Testing:
- Person seeks testing of their own free will
- Fully voluntary, confidential
2. Provider-Initiated Testing and Counselling (PITC):
- Medical provider advises/recommends testing
- Routine offer with "opt-out" option
- Used in ANC clinics, TB patients, STD clinics
WHO Five C's Governing All HIV Testing:
- Consent - informed, voluntary
- Confidentiality - results protected
- Counselling - pre and post-test
- Correct test results - quality assured
- Connection - linkage to care and treatment
SLIDE 4 - HIV Testing Strategies (Continued)
Testing Approaches by Population
| Population | Testing Strategy |
|---|
| High-risk groups (IDU, MSM, FSW, TG) | Unlinked anonymous with informed consent |
| Bridge population (migrants, truckers) | Unlinked anonymous at STD clinics |
| Pregnant women (ANC) | Routine opt-out testing |
| TB patients | PITC - routine HIV testing |
- Sentinel surveillance conducted once in 2 years
- Sample size: 250 (high-risk), 400 (ANC)
- Testing protocol: Two-test protocol
SLIDE 5 - ICTC: Introduction
Integrated Counselling and Testing Centre (ICTC)
- Primary facility for HIV counselling and testing in India
- A person is counselled and tested either:
- Client-initiated (of own free will), or
- Provider-initiated (advised by medical provider)
Functions of ICTC:
- Early detection of HIV
- Provision of basic information on modes of transmission and prevention
- Promoting behavioural change and reducing vulnerability
- Linking PLHIV with HIV prevention, care and treatment services
Scale: More than 15,000 ICTCs across India (as of recent data)
SLIDE 6 - Types of ICTC Facilities
Classification of ICTCs
1. Fixed Facility ICTCs
a. Standalone ICTC (SA-ICTC):
- High client load
- Full-time counsellor + laboratory technician
- Located in medical colleges, district hospitals, sub-district hospitals, CHCs
b. Facility-Integrated ICTC (F-ICTC):
- Set up at block level (24x7 PHCs)
- Existing health facility staff trained in counselling and testing
- Logistic support from DAC (Department of AIDS Control)
- Public-Private Partnership (PPP)-ICTCs also established
2. Mobile ICTC:
- Van with room for examination, counselling, blood sample collection
- Team: Health educator/ANM + Counsellor + Laboratory technician
- Serves hard-to-reach areas with flexible hours
- Provides: HIV testing, STI/RTI syndromic management, ANC, immunization
SLIDE 7 - HIV Counselling
Pre-Test Counselling
Conducted before the HIV test. Key components:
- Explanation of the purpose and nature of the test
- Information on HIV transmission modes and prevention
- Discussion of risk behaviours
- Implications of a positive vs negative result
- Obtaining informed consent
- Addressing fears, stigma, confidentiality concerns
- Preparing the client emotionally for results
Key principle: Testing is a gateway to HIV prevention, treatment, care and support services
SLIDE 8 - HIV Counselling
Post-Test Counselling
Conducted after the result is given. Differs by result:
If HIV Negative:
- Reinforce risk reduction behaviours
- Discuss window period (need for re-testing if recent exposure)
- Encourage continued safe practices
If HIV Positive:
- Emotional support and disclosure counselling
- Information on disease progression, treatment options
- Referral to ART centre and other PLHIV support services
- Partner notification and testing guidance
- Positive living advice (nutrition, safe sex, adherence)
- Linkage to PPTCT services (if pregnant)
SLIDE 9 - Diagnostic Tests for HIV
Laboratory Tests & Their Significance
| Test | Purpose & Significance |
|---|
| HIV ELISA | Screening test; Sensitivity >99.9%; Repeatedly reactive results must be confirmed |
| Western Blot | Confirmatory test; Specificity >99.99% when combined with ELISA; Indeterminate in early HIV, HIV-2, autoimmune disease, pregnancy |
| Absolute CD4 count | Most widely used predictor of HIV progression; Risk of OI high if CD4 <200 cells/μL |
| CD4 lymphocyte % | More reliable than absolute count; Risk high if <14% |
| HIV Viral Load | Measures actively replicating HIV; Correlates with disease progression and ART response |
| p24 Antigen | Indicates active HIV replication; Positive before seroconversion and with advanced disease |
| β2-Microglobulin | Macrophage-monocyte stimulation marker; Level >3.5 mg/dL = rapid progression |
| CBC | Anaemia, neutropenia, thrombocytopenia common with advanced HIV |
SLIDE 10 - Diagnostic Tests: Testing Protocol
Standard Testing Algorithm in India
- Two-test protocol used for sentinel surveillance
- Screening with ELISA (high sensitivity)
- Reactive samples confirmed with Western Blot (high specificity)
- Rapid tests available for point-of-care testing at ICTCs
- Dried Blood Spot (DBS) specimens used at TI project sites
Window Period:
- Period between infection and detectable antibodies
- Usually 2-12 weeks (most within 6 weeks)
- Person may be infectious but test negative
- Re-testing advised if recent high-risk exposure
SLIDE 11 - HIV Screening in Special Populations
1. Pregnant Women (PPTCT Programme)
- PPTCT (Prevention of Parent-to-Child Transmission) started in India in 2002
- Routine offer of HIV counselling and testing to all pregnant women with "opt-out" option
- Spouse involvement encouraged
- Evolution: Single-dose NVP (2002) → Multi-drug ARV (2012) → Lifelong ART "Option B+" (2013 onwards)
- Lifelong ART for all HIV+ pregnant and breastfeeding women regardless of CD4 count or WHO clinical stage
- Goal: Eliminate mother-to-child transmission of HIV
SLIDE 12 - HIV Screening in Special Populations
2. TB Patients
- HIV testing of TB patients jointly implemented by NACP and RNTCP since 2007-08
- PITC used routinely through the intensified TB-HIV package
- States with high HIV prevalence cover ~90% TB patients for HIV testing
- HIV testing offered upstream during evaluation for TB symptoms (since October 2012)
- Expedites HIV detection within 2-4 weeks of TB positivity
- Case fatality rate among HIV-TB co-infected patients: 13-14% vs <4% in HIV-negative TB cases
3. HIV-Exposed Infants
- All infants born to HIV-positive mothers screened
- Early Infant Diagnosis (EID) at 6 weeks using DNA PCR
SLIDE 13 - HIV Screening in Special Populations
4. High-Risk Groups (HRG) and Bridge Populations
| Group | Strategy |
|---|
| Injecting Drug Users (IDU) | Targeted interventions (TI), unlinked anonymous testing |
| Men who have Sex with Men (MSM) | TI projects, sentinel surveillance |
| Female Sex Workers (FSW) | TI projects |
| Transgender (TG) | TI projects |
| Single Male Migrants (SMM) | STD clinic-based testing |
| Long Distance Truckers (LDT) | TI project-based testing |
| STD clinic attendees | Syndromic management + HIV testing |
5. Community-Based HIV Screening
- Frontline health workers (ANMs) conduct testing at sub-centre level
- Aims to offer HIV testing to every pregnant woman in the country
SLIDE 14 - HIV Testing Services: Hierarchy in India
Levels of HIV Counselling and Testing Services:
- State/District Level - Standalone ICTCs at medical colleges, district hospitals
- Sub-District Level - F-ICTCs at sub-district hospitals, CHCs
- Block Level - F-ICTCs at 24x7 PHCs; PPP-ICTCs
- Village/Community Level - Mobile ICTCs, community-based screening by ANMs
SLIDE 15 - Summary
Key Takeaways
- HIV testing is voluntary, confidential and guided by WHO 5 C's
- Two main strategies: VCT (client-initiated) and PITC (provider-initiated)
- ICTC is the backbone of HIV testing in India - Fixed (Standalone/Facility-integrated) and Mobile
- Counselling is integral - pre-test (consent, risk assessment) and post-test (support, linkage)
- Diagnosis uses ELISA (screening) followed by Western Blot (confirmation); CD4 count guides prognosis
- Special population screening: Pregnant women (PPTCT), TB patients (PITC), HRGs (targeted interventions), infants born to HIV+ mothers
- Test and Treat policy and 90-90-90 targets drive the national programme
All content sourced from: Park's Textbook of Preventive and Social Medicine (Park's PSM), Department of AIDS Control / NACP guidelines
Notes for your presentation:
- The slides follow the exact order of your assigned topics
- Slide 9 has the full diagnostic tests table - great for quick recall during viva
- Slide 11-13 cover all special populations comprehensively
- You can split Slide 6 and Slide 9 into two visual slides each for better readability