Here are the answers with brief explanations:
2. Robertson cooked meat broth → c. Anaerobic media
Robertson's cooked meat (RCM) broth is a classic anaerobic medium. The meat particles absorb oxygen and provide a low redox environment, supporting growth of anaerobes like Clostridium spp.
3. MRSA resistance is mediated by → b. MecA gene
The mecA gene (carried on the mobile genetic element SCCmec) encodes an altered penicillin-binding protein, PBP2a (PBP2'), which has very low affinity for all beta-lactam antibiotics, conferring broad resistance.
4. T cell-dependent antigen → d. Exotoxin
Exotoxins are protein antigens that require T cell help (via MHC class II presentation) to stimulate B cells to produce antibody. Bacterial capsule polysaccharides, flagella, and LPS are T cell-independent antigens (they can directly stimulate B cells without T cell help).
5. Splenic defect predisposes to all EXCEPT → a. Staphylococcus aureus
The spleen is critical for opsonization and clearance of encapsulated organisms. Asplenic patients are classically susceptible to Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae (the "SHiN" organisms). S. aureus is not primarily an encapsulated organism and its clearance does not depend critically on splenic function.
6. Five moments of hand hygiene - all EXCEPT → d. Before touching patient's surroundings
The WHO 5 Moments are:
- Before touching a patient
- Before a clean/aseptic procedure
- After body fluid exposure risk
- After touching a patient
- After touching patient surroundings
"Before touching patient's surroundings" is not one of the 5 moments (only "after" touching surroundings is included).
7. Ideal concentration method for Ascaris unfertilized eggs → a. Formalin ethyl acetate sedimentation
Unfertilized Ascaris eggs are heavy and do not float well in zinc sulfate or saturated salt/sugar flotation methods (their specific gravity is too high). Formalin-ethyl acetate (FEA) sedimentation is the method of choice as it recovers these dense, atypical eggs reliably.