ls -la /home/daytona/workspace/ && mkdir -p /home/daytona/workspace/synapse-pptx

~/synapse-pptx/build.js

const pptxgen = require("pptxgenjs");

const pres = new pptxgen();
pres.layout = "LAYOUT_16x9";
pres.title = "Synapse: Classification & Predestination";
pres.author = "Medical Education";

// ─── THEME ────────────────────────────────────────────────────────────────────
const BG       = "0D1B2A";   // deep navy – dominates
const ACCENT   = "00C9A7";   // teal-green – sharp accent
const ACCENT2  = "1A78C2";   // mid-blue – supporting
const LIGHT    = "E8F4FD";   // near-white text
const MID      = "A8C8E8";   // subdued text / labels
const GOLD     = "F4C430";   // highlight / key term

// helper – dark bg slide
function addSlide(bg) {
  const s = pres.addSlide();
  s.addShape(pres.ShapeType.rect, { x: 0, y: 0, w: 10, h: 5.625, fill: { color: bg || BG }, line: { type: "none" } });
  return s;
}

// header bar helper
function header(s, title, sub) {
  s.addShape(pres.ShapeType.rect, { x: 0, y: 0, w: 10, h: 0.85, fill: { color: ACCENT2 }, line: { type: "none" } });
  s.addText(title, { x: 0.3, y: 0, w: 9.4, h: 0.85, fontSize: 22, bold: true, color: "FFFFFF", valign: "middle", fontFace: "Calibri", margin: 0 });
  if (sub) s.addText(sub, { x: 0.3, y: 0.82, w: 9.4, h: 0.32, fontSize: 11, color: ACCENT, italic: true, fontFace: "Calibri", margin: 0 });
}

// bullet helper
function bullets(s, items, x, y, w, h, opts) {
  const arr = items.map((item, i) => ({
    text: item,
    options: { bullet: { code: "25B6", indent: 10, color: ACCENT }, color: LIGHT, fontSize: opts && opts.fontSize ? opts.fontSize : 14, fontFace: "Calibri", breakLine: i < items.length - 1 }
  }));
  s.addText(arr, { x, y, w, h, valign: "top", margin: [4, 4, 4, 4] });
}

// accent box helper
function accentBox(s, text, x, y, w, h, bg, fc, fs) {
  s.addShape(pres.ShapeType.rect, { x, y, w, h, fill: { color: bg || ACCENT2 }, line: { color: ACCENT, pt: 1.5 }, rectRadius: 0.1 });
  s.addText(text, { x, y, w, h, fontSize: fs || 12, color: fc || "FFFFFF", bold: true, align: "center", valign: "middle", fontFace: "Calibri", margin: 0 });
}

// ─── SLIDE 1 – TITLE ──────────────────────────────────────────────────────────
{
  const s = addSlide();
  // gradient strip
  s.addShape(pres.ShapeType.rect, { x: 0, y: 4.2, w: 10, h: 1.425, fill: { color: ACCENT2 }, line: { type: "none" } });
  s.addShape(pres.ShapeType.rect, { x: 0, y: 0, w: 0.15, h: 5.625, fill: { color: ACCENT }, line: { type: "none" } });

  s.addText("SYNAPSE", { x: 0.4, y: 0.5, w: 9.2, h: 1.1, fontSize: 56, bold: true, color: ACCENT, align: "left", fontFace: "Calibri", charSpacing: 8, margin: 0 });
  s.addText("Classification & Predestination", { x: 0.4, y: 1.55, w: 9.2, h: 0.7, fontSize: 26, color: LIGHT, align: "left", fontFace: "Calibri", margin: 0 });
  s.addText("For Medical & Nursing Students", { x: 0.4, y: 2.3, w: 9.2, h: 0.45, fontSize: 16, color: MID, align: "left", italic: true, fontFace: "Calibri", margin: 0 });

  s.addText([
    { text: "▸ Definitions & anatomy of the synapse", options: { color: LIGHT, fontSize: 13, breakLine: true } },
    { text: "▸ Electrical vs. chemical synapses", options: { color: LIGHT, fontSize: 13, breakLine: true } },
    { text: "▸ Structural classification (axodendritic, axosomatic…)", options: { color: LIGHT, fontSize: 13, breakLine: true } },
    { text: "▸ Functional classification (excitatory / inhibitory)", options: { color: LIGHT, fontSize: 13, breakLine: true } },
    { text: "▸ Synaptic predestination & directional specificity", options: { color: LIGHT, fontSize: 13 } }
  ], { x: 0.4, y: 2.85, w: 5.5, h: 1.35, valign: "top", margin: 0 });

  s.addText("Neuroscience: Exploring the Brain, 5e  |  Guyton & Hall Medical Physiology", { x: 0.4, y: 4.3, w: 9, h: 0.35, fontSize: 10, color: LIGHT, italic: true, margin: 0 });
}

// ─── SLIDE 2 – WHAT IS A SYNAPSE? ─────────────────────────────────────────────
{
  const s = addSlide();
  header(s, "What Is a Synapse?", "Definition & Historical Context");

  s.addText([
    { text: "A synapse ", options: { bold: true, color: GOLD, fontSize: 15 } },
    { text: "is the specialized junction where one part of a neuron contacts and communicates with another neuron or non-neural cell (muscle, gland).", options: { color: LIGHT, fontSize: 15 } }
  ], { x: 0.35, y: 1.0, w: 9.3, h: 0.7, valign: "middle", fontFace: "Calibri", margin: 0 });

  const keyFacts = [
    "Term coined by Sir Charles Sherrington (1897) from Greek syn (together) + haptein (to clasp)",
    "Transfer of information at the synapse is called synaptic transmission",
    "Two fundamental types: Electrical and Chemical",
    "Direction of information flow defines sides: Presynaptic → Postsynaptic",
    "Synaptic transmission dysfunction underlies many neurological and psychiatric disorders"
  ];
  bullets(s, keyFacts, 0.35, 1.8, 6.0, 3.5, { fontSize: 13.5 });

  // side info box
  s.addShape(pres.ShapeType.rect, { x: 6.6, y: 1.1, w: 3.1, h: 3.9, fill: { color: "13263B" }, line: { color: ACCENT, pt: 1.5 } });
  s.addText("Key Terms", { x: 6.65, y: 1.15, w: 3.0, h: 0.4, fontSize: 12, bold: true, color: ACCENT, align: "center", fontFace: "Calibri", margin: 0 });
  const kts = [
    ["Presynaptic", "Initiates signal"],
    ["Postsynaptic", "Receives signal"],
    ["Synaptic cleft", "20–50 nm gap"],
    ["Active zone", "NT release site"],
    ["PSD", "Postsynaptic density"],
  ];
  kts.forEach(([term, def], i) => {
    s.addText([
      { text: term + ": ", options: { bold: true, color: GOLD } },
      { text: def, options: { color: LIGHT } }
    ], { x: 6.7, y: 1.6 + i * 0.62, w: 2.95, h: 0.5, fontSize: 11, fontFace: "Calibri", margin: 0 });
  });
}

// ─── SLIDE 3 – ANATOMY OF THE SYNAPSE ─────────────────────────────────────────
{
  const s = addSlide();
  header(s, "Anatomical Structure of the Chemical Synapse", "Structural components");

  const parts = [
    ["Presynaptic Terminal", "Axon bouton containing synaptic vesicles, mitochondria, and active zones"],
    ["Synaptic Vesicles", "Small (~50 nm) membrane-enclosed spheres storing neurotransmitter"],
    ["Dense-Core Vesicles", "Larger (~100 nm); store peptide neurotransmitters / secretory granules"],
    ["Synaptic Cleft", "20–50 nm gap filled with fibrous extracellular protein matrix (acts as glue)"],
    ["Active Zone", "Presynaptic membrane proteins from which vesicles undergo exocytosis"],
    ["Postsynaptic Density (PSD)", "Thick protein accumulation beneath postsynaptic membrane containing neurotransmitter receptors"],
    ["Postsynaptic Membrane", "Contains ionotropic or metabotropic receptors that transduce chemical signal"]
  ];

  parts.forEach(([term, desc], i) => {
    const col = i % 2 === 0 ? "13263B" : "0A1E30";
    s.addShape(pres.ShapeType.rect, { x: 0.2, y: 0.95 + i * 0.65, w: 9.6, h: 0.6, fill: { color: col }, line: { type: "none" } });
    s.addText([
      { text: term + "  ", options: { bold: true, color: GOLD, fontSize: 12 } },
      { text: desc, options: { color: MID, fontSize: 11.5 } }
    ], { x: 0.35, y: 0.95 + i * 0.65, w: 9.3, h: 0.6, valign: "middle", fontFace: "Calibri", margin: 0 });
  });

  s.addText("Source: Neuroscience – Exploring the Brain, 5e", { x: 0.35, y: 5.35, w: 9, h: 0.25, fontSize: 9, color: MID, italic: true, margin: 0 });
}

// ─── SLIDE 4 – ELECTRICAL SYNAPSES ────────────────────────────────────────────
{
  const s = addSlide();
  header(s, "Electrical Synapses", "Gap junctions – direct ionic coupling");

  bullets(s, [
    "Formed at gap junctions – membranes separated by only ~3 nm (vs 20–50 nm in chemical synapses)",
    "Bridged by connexin proteins → 6 connexins form a connexon; two connexons form a gap junction channel",
    "~20 subtypes of connexins; ~half found in the brain",
    "Channel pore ~1–2 nm: large enough for major ions and small organic molecules",
    "Bidirectional transmission – signals pass in both directions (unlike chemical synapses)",
    "Fast & synchronous – no delay of neurotransmitter diffusion",
    "Found between: dendrites, cell bodies, occasionally axons; also in cardiac/smooth muscle, glia, epithelium"
  ], 0.35, 0.95, 5.8, 4.3, { fontSize: 13 });

  // function boxes
  const fns = ["Synchronise large neuron groups", "Detect coincident subthreshold depolarisations", "Coordinate hypothalamic hormone secretion", "Enable rapid escape responses"];
  fns.forEach((f, i) => {
    accentBox(s, f, 6.25, 1.05 + i * 0.97, 3.5, 0.82, "0D2840", ACCENT, 11);
  });
  s.addText("Functions", { x: 6.25, y: 0.9, w: 3.5, h: 0.4, fontSize: 12, bold: true, color: GOLD, align: "center", fontFace: "Calibri", margin: 0 });
  s.addText("Source: Guyton & Hall, 14e  |  Neuroscience – Exploring the Brain, 5e", { x: 0.35, y: 5.35, w: 9, h: 0.25, fontSize: 9, color: MID, italic: true, margin: 0 });
}

// ─── SLIDE 5 – CHEMICAL SYNAPSES ──────────────────────────────────────────────
{
  const s = addSlide();
  header(s, "Chemical Synapses", "Neurotransmitter-mediated signal transduction");

  const steps = [
    ["1", "Action potential reaches presynaptic terminal"],
    ["2", "Membrane depolarisation opens voltage-gated Ca²⁺ channels"],
    ["3", "Ca²⁺ influx → vesicle fusion at active zones (exocytosis)"],
    ["4", "Neurotransmitter diffuses across synaptic cleft (20–50 nm)"],
    ["5", "NT binds postsynaptic receptors → ion channel opening or 2nd messenger cascade"],
    ["6", "NT cleared: reuptake, enzymatic degradation, or diffusion"]
  ];

  steps.forEach(([n, text], i) => {
    s.addShape(pres.ShapeType.rect, { x: 0.2, y: 1.0 + i * 0.73, w: 0.55, h: 0.6, fill: { color: ACCENT }, line: { type: "none" } });
    s.addText(n, { x: 0.2, y: 1.0 + i * 0.73, w: 0.55, h: 0.6, fontSize: 16, bold: true, color: BG, align: "center", valign: "middle", fontFace: "Calibri", margin: 0 });
    s.addText(text, { x: 0.85, y: 1.0 + i * 0.73, w: 8.8, h: 0.6, fontSize: 13.5, color: LIGHT, valign: "middle", fontFace: "Calibri", margin: 0 });
  });

  // key characteristics bar
  s.addShape(pres.ShapeType.rect, { x: 0, y: 5.15, w: 10, h: 0.475, fill: { color: ACCENT2 }, line: { type: "none" } });
  s.addText("One-way conduction  |  Amplification of signal  |  Modifiable (plasticity)  |  Delay ~0.5–2 ms  |  Most synapses in the CNS are chemical",
    { x: 0.3, y: 5.15, w: 9.4, h: 0.475, fontSize: 11, color: "FFFFFF", align: "center", valign: "middle", fontFace: "Calibri", margin: 0 });
}

// ─── SLIDE 6 – ELECTRICAL vs CHEMICAL ─────────────────────────────────────────
{
  const s = addSlide();
  header(s, "Electrical vs. Chemical Synapses: Comparison", "Two fundamental synapse types");

  const rows = [
    ["Feature", "Electrical Synapse", "Chemical Synapse"],
    ["Structural gap", "~3 nm (gap junction)", "20–50 nm (synaptic cleft)"],
    ["Direction of transmission", "Bidirectional (usually)", "Unidirectional only"],
    ["Speed", "Extremely fast (no delay)", "Delay ~0.5–2 ms"],
    ["Signal amplification", "None (1:1 ionic current)", "Yes (cascade amplification)"],
    ["Modifiability", "Limited", "Highly modifiable (plasticity)"],
    ["Mediator", "Ions through connexons", "Neurotransmitter + receptors"],
    ["Example location", "Cardiac muscle, CNS interneurons", "Most CNS & PNS synapses"],
    ["Synchronisation", "Excellent", "Poor"],
  ];

  rows.forEach(([a, b, c], i) => {
    const isHeader = i === 0;
    const bg = isHeader ? ACCENT2 : (i % 2 === 0 ? "13263B" : "0A1E30");
    const fc = isHeader ? "FFFFFF" : LIGHT;
    const fs = isHeader ? 12 : 11.5;
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    s.addShape(pres.ShapeType.rect, { x: 3.2,  y: 0.92 + i * 0.51, w: 3.0,  h: 0.48, fill: { color: isHeader ? ACCENT2 : bg }, line: { color: isHeader ? ACCENT : "1A3050", pt: 0.5 } });
    s.addShape(pres.ShapeType.rect, { x: 6.3,  y: 0.92 + i * 0.51, w: 3.5,  h: 0.48, fill: { color: isHeader ? ACCENT2 : bg }, line: { color: isHeader ? ACCENT : "1A3050", pt: 0.5 } });
    [[a, 0.15, 2.95], [b, 3.2, 3.0], [c, 6.3, 3.5]].forEach(([txt, x, w]) => {
      s.addText(txt, { x, y: 0.92 + i * 0.51, w, h: 0.48, fontSize: fs, bold: isHeader, color: isHeader ? "FFFFFF" : (a === "Feature" ? GOLD : fc), align: "center", valign: "middle", fontFace: "Calibri", margin: 0 });
    });
  });
}

// ─── SLIDE 7 – STRUCTURAL CLASSIFICATION ──────────────────────────────────────
{
  const s = addSlide();
  header(s, "Structural Classification of Synapses", "Named by pre→post contact site");

  const types = [
    { name: "Axodendritic", icon: "A→D", desc: "Axon terminal contacts a dendrite. Most common synapse in the CNS. Modulates integration of inputs on dendritic tree.", color: ACCENT },
    { name: "Axosomatic", icon: "A→S", desc: "Axon terminal contacts the cell body (soma). Often inhibitory; powerfully controls neuronal firing threshold.", color: ACCENT2 },
    { name: "Axoaxonic", icon: "A→A", desc: "Axon terminal contacts another axon. Mediates presynaptic inhibition/facilitation by modulating NT release from the postsynaptic axon.", color: GOLD },
    { name: "Axospinous", icon: "A→Sp", desc: "Axon contacts a dendritic spine specifically. Site of many excitatory synapses; key substrate for synaptic plasticity (LTP).", color: "FF6B6B" },
    { name: "Dendrodendritic", icon: "D↔D", desc: "Dendrite-to-dendrite synapse. Found in olfactory bulb and retina; can be bidirectional, mediating local circuit processing.", color: "C77DFF" },
  ];

  types.forEach((t, i) => {
    const bx = (i % 2) * 4.9 + 0.2;
    const by = Math.floor(i / 2) * 1.65 + 1.0;
    const isLast = i === 4;
    const bxF = isLast ? 2.65 : bx;

    s.addShape(pres.ShapeType.rect, { x: bxF, y: by, w: 4.7, h: 1.5, fill: { color: "0A1E30" }, line: { color: t.color, pt: 2 }, rectRadius: 0.12 });
    s.addShape(pres.ShapeType.rect, { x: bxF, y: by, w: 1.1, h: 1.5, fill: { color: t.color }, line: { type: "none" } });
    s.addText(t.icon, { x: bxF, y: by, w: 1.1, h: 1.5, fontSize: 14, bold: true, color: BG, align: "center", valign: "middle", fontFace: "Calibri", margin: 0 });
    s.addText(t.name, { x: bxF + 1.15, y: by + 0.05, w: 3.45, h: 0.42, fontSize: 13, bold: true, color: t.color, fontFace: "Calibri", margin: 0 });
    s.addText(t.desc, { x: bxF + 1.15, y: by + 0.42, w: 3.45, h: 1.0, fontSize: 10.5, color: LIGHT, fontFace: "Calibri", valign: "top", margin: 0 });
  });
}

// ─── SLIDE 8 – FUNCTIONAL CLASSIFICATION ──────────────────────────────────────
{
  const s = addSlide();
  header(s, "Functional Classification: Excitatory vs. Inhibitory", "Effect on postsynaptic membrane potential");

  // Excitatory panel
  s.addShape(pres.ShapeType.rect, { x: 0.2, y: 1.0, w: 4.6, h: 4.35, fill: { color: "0A2010" }, line: { color: "00C853", pt: 2 } });
  s.addText("EXCITATORY SYNAPSE", { x: 0.35, y: 1.05, w: 4.3, h: 0.5, fontSize: 13, bold: true, color: "00C853", align: "center", fontFace: "Calibri", margin: 0 });
  s.addShape(pres.ShapeType.line, { x: 0.35, y: 1.55, w: 4.3, h: 0, line: { color: "00C853", pt: 1 } });
  const exPoints = [
    "Generates EPSP (Excitatory Postsynaptic Potential)",
    "Increases Na⁺ / Ca²⁺ conductance → membrane depolarisation",
    "Moves Vm toward threshold (~−55 mV)",
    "Key NTs: Glutamate (AMPA/NMDA), Acetylcholine (nicotinic)",
    "Typically axodendritic or axospinous location",
    "Gray type I synapses – wide cleft, thick PSD",
    "Summation of EPSPs triggers action potential"
  ];
  bullets(s, exPoints, 0.3, 1.6, 4.4, 3.6, { fontSize: 11.5 });

  // Inhibitory panel
  s.addShape(pres.ShapeType.rect, { x: 5.2, y: 1.0, w: 4.6, h: 4.35, fill: { color: "200A0A" }, line: { color: "FF4444", pt: 2 } });
  s.addText("INHIBITORY SYNAPSE", { x: 5.35, y: 1.05, w: 4.3, h: 0.5, fontSize: 13, bold: true, color: "FF4444", align: "center", fontFace: "Calibri", margin: 0 });
  s.addShape(pres.ShapeType.line, { x: 5.35, y: 1.55, w: 4.3, h: 0, line: { color: "FF4444", pt: 1 } });
  const inhPoints = [
    "Generates IPSP (Inhibitory Postsynaptic Potential)",
    "Increases Cl⁻ or K⁺ conductance → hyperpolarisation",
    "Moves Vm away from threshold (more negative)",
    "Key NTs: GABA (GABA-A/B receptors), Glycine",
    "Often axosomatic location – strong control of firing",
    "Gray type II synapses – narrow cleft, thin PSD",
    "Shunting inhibition: reduces EPSP amplitude"
  ];
  bullets(s, inhPoints, 5.3, 1.6, 4.4, 3.6, { fontSize: 11.5 });
}

// ─── SLIDE 9 – NEUROTRANSMITTERS ──────────────────────────────────────────────
{
  const s = addSlide();
  header(s, "Neurotransmitters: Classification & Examples", "Chemical mediators of synaptic transmission");

  const cats = [
    {
      cat: "Amino Acids", color: ACCENT,
      items: ["Glutamate – major excitatory NT of CNS (AMPA, NMDA, Kainate receptors)", "GABA – major inhibitory NT of CNS (GABA-A, GABA-B)", "Glycine – inhibitory NT in spinal cord & brainstem", "Aspartate – excitatory co-transmitter"]
    },
    {
      cat: "Biogenic Amines", color: GOLD,
      items: ["Acetylcholine (ACh) – NMJ, ANS, basal forebrain", "Dopamine – reward, motor control (nigrostriatal)", "Serotonin (5-HT) – mood, sleep, cognition", "Norepinephrine – arousal, autonomic", "Histamine – hypothalamic modulation"]
    },
    {
      cat: "Peptides", color: "C77DFF",
      items: ["Substance P – pain transmission", "Endorphins/Enkephalins – pain modulation", "Neuropeptide Y – energy balance", "Stored in dense-core vesicles; released under high-frequency stimulation"]
    }
  ];

  cats.forEach((c, i) => {
    const bx = i * 3.25 + 0.2;
    s.addShape(pres.ShapeType.rect, { x: bx, y: 1.0, w: 3.1, h: 4.35, fill: { color: "0A1E30" }, line: { color: c.color, pt: 2 } });
    s.addShape(pres.ShapeType.rect, { x: bx, y: 1.0, w: 3.1, h: 0.55, fill: { color: c.color }, line: { type: "none" } });
    s.addText(c.cat, { x: bx, y: 1.0, w: 3.1, h: 0.55, fontSize: 13, bold: true, color: BG, align: "center", valign: "middle", fontFace: "Calibri", margin: 0 });
    bullets(s, c.items, bx + 0.08, 1.6, 2.94, 3.6, { fontSize: 11 });
  });
}

// ─── SLIDE 10 – SYNAPTIC PREDESTINATION ───────────────────────────────────────
{
  const s = addSlide();
  header(s, "Synaptic Predestination", "Directional specificity of chemical synapses");

  s.addShape(pres.ShapeType.rect, { x: 0.2, y: 1.0, w: 9.6, h: 1.05, fill: { color: "102030" }, line: { color: GOLD, pt: 2 } });
  s.addText([
    { text: "Definition: ", options: { bold: true, color: GOLD, fontSize: 14 } },
    { text: "Synaptic predestination refers to the inherent, structurally fixed directionality of chemical synaptic transmission — signals can only flow from the presynaptic neuron to the postsynaptic neuron, never in reverse.", options: { color: LIGHT, fontSize: 13.5 } }
  ], { x: 0.35, y: 1.0, w: 9.3, h: 1.05, valign: "middle", fontFace: "Calibri", margin: 0 });

  const reasons = [
    ["Asymmetric structure", "Synaptic vesicles and active zones are exclusively on the presynaptic side; receptors only on the postsynaptic side"],
    ["Neurotransmitter storage", "NT is packaged in vesicles at the presynaptic terminal — postsynaptic membrane has no vesicles to release NT retrogradely"],
    ["Receptor distribution", "Neurotransmitter receptors are clustered in the postsynaptic density (PSD); absent or sparse presynaptically"],
    ["Active zone machinery", "Ca²⁺-triggered exocytosis machinery (SNARE proteins, RIM, Munc13) is restricted to the presynaptic terminal"],
    ["Diffusion geometry", "NT is released focally into the cleft and diffuses toward the postsynaptic density — not away from it"],
  ];

  reasons.forEach(([title, desc], i) => {
    s.addShape(pres.ShapeType.rect, { x: 0.2, y: 2.15 + i * 0.67, w: 2.3, h: 0.6, fill: { color: ACCENT2 }, line: { type: "none" } });
    s.addText(title, { x: 0.2, y: 2.15 + i * 0.67, w: 2.3, h: 0.6, fontSize: 11, bold: true, color: "FFFFFF", align: "center", valign: "middle", fontFace: "Calibri", margin: 0 });
    s.addShape(pres.ShapeType.rect, { x: 2.6, y: 2.15 + i * 0.67, w: 7.2, h: 0.6, fill: { color: i % 2 === 0 ? "0D2035" : "0A1A2B" }, line: { type: "none" } });
    s.addText(desc, { x: 2.7, y: 2.15 + i * 0.67, w: 7.0, h: 0.6, fontSize: 12, color: LIGHT, valign: "middle", fontFace: "Calibri", margin: 0 });
  });
}

// ─── SLIDE 11 – WHY PREDESTINATION MATTERS ────────────────────────────────────
{
  const s = addSlide();
  header(s, "Why Synaptic Predestination Matters", "Functional significance & clinical relevance");

  const points = [
    {
      title: "Directed Signal Routing",
      desc: "One-way conduction enables signals to be directed to specific targets — allows the nervous system to perform sensation, motor control, memory, and countless other functions without 'noise' from reverse transmission.",
      col: ACCENT
    },
    {
      title: "Neural Circuit Architecture",
      desc: "Predestination allows construction of feed-forward and feed-back circuits, reflex arcs, and complex networks with defined information flow.",
      col: ACCENT2
    },
    {
      title: "Pharmacological Targeting",
      desc: "Because NT release, receptor binding, and clearance are pre-destined to specific sides, drugs can selectively target presynaptic (e.g. reserpine, MAOIs) or postsynaptic (e.g. receptor antagonists, antipsychotics) mechanisms.",
      col: GOLD
    },
    {
      title: "Synaptic Amplification",
      desc: "A small bolus of NT from a chemical synapse can trigger an amplifying cascade of molecular events on the postsynaptic side — this amplification would be lost if signalling were bidirectional.",
      col: "C77DFF"
    }
  ];

  points.forEach((p, i) => {
    const bx = (i % 2) * 4.9 + 0.15;
    const by = Math.floor(i / 2) * 2.1 + 1.0;
    s.addShape(pres.ShapeType.rect, { x: bx, y: by, w: 4.7, h: 1.95, fill: { color: "0A1E30" }, line: { color: p.col, pt: 2 } });
    s.addShape(pres.ShapeType.rect, { x: bx, y: by, w: 4.7, h: 0.5, fill: { color: p.col }, line: { type: "none" } });
    s.addText(p.title, { x: bx + 0.1, y: by, w: 4.5, h: 0.5, fontSize: 12.5, bold: true, color: BG, align: "center", valign: "middle", fontFace: "Calibri", margin: 0 });
    s.addText(p.desc, { x: bx + 0.1, y: by + 0.55, w: 4.5, h: 1.35, fontSize: 11, color: LIGHT, valign: "top", fontFace: "Calibri", margin: 0 });
  });
}

// ─── SLIDE 12 – PRESYNAPTIC INHIBITION ────────────────────────────────────────
{
  const s = addSlide();
  header(s, "Presynaptic Inhibition & Facilitation", "Axoaxonic synapses modulate predestined pathways");

  s.addText([
    { text: "Presynaptic inhibition ", options: { bold: true, color: GOLD, fontSize: 14 } },
    { text: "is a mechanism by which an axoaxonic synapse reduces neurotransmitter release from the target axon — modulating information flow WITHOUT changing the postsynaptic neuron's threshold.", options: { color: LIGHT, fontSize: 13 } }
  ], { x: 0.3, y: 1.0, w: 9.4, h: 0.9, valign: "middle", fontFace: "Calibri", margin: 0 });

  const mechRows = [
    ["Mechanism", "Detail"],
    ["GABA-B receptor activation", "G-protein coupled → ↓ Ca²⁺ channel opening → less Ca²⁺ influx → fewer vesicles release NT"],
    ["K⁺ channel opening", "Axon terminal hyperpolarisation shortens action potential duration → less NT release"],
    ["Direct inhibition of exocytosis", "GABA-B can also directly inhibit the vesicle fusion machinery"],
    ["Net effect", "Reduces EPSP amplitude at the postsynaptic neuron without directly acting on it"],
    ["Presynaptic facilitation", "Opposite: e.g. serotonin-mediated ↑ Ca²⁺ → enhanced NT release at target synapse"],
  ];

  mechRows.forEach(([a, b], i) => {
    const isH = i === 0;
    const bg = isH ? ACCENT2 : (i % 2 === 0 ? "0D2035" : "0A1A2B");
    s.addShape(pres.ShapeType.rect, { x: 0.2, y: 2.0 + i * 0.54, w: 3.2, h: 0.5, fill: { color: bg }, line: { color: "1A3050", pt: 0.5 } });
    s.addShape(pres.ShapeType.rect, { x: 3.5, y: 2.0 + i * 0.54, w: 6.3, h: 0.5, fill: { color: bg }, line: { color: "1A3050", pt: 0.5 } });
    s.addText(a, { x: 0.25, y: 2.0 + i * 0.54, w: 3.1, h: 0.5, fontSize: isH ? 12 : 11, bold: isH, color: isH ? "FFFFFF" : GOLD, valign: "middle", fontFace: "Calibri", margin: 0 });
    s.addText(b, { x: 3.55, y: 2.0 + i * 0.54, w: 6.15, h: 0.5, fontSize: isH ? 12 : 11, bold: isH, color: isH ? "FFFFFF" : LIGHT, valign: "middle", fontFace: "Calibri", margin: 0 });
  });

  s.addShape(pres.ShapeType.rect, { x: 0.2, y: 5.1, w: 9.6, h: 0.35, fill: { color: "102030" }, line: { type: "none" } });
  s.addText("Clinical note: Baclofen (GABA-B agonist) exploits presynaptic inhibition at spinal cord synapses to treat spasticity.",
    { x: 0.3, y: 5.1, w: 9.3, h: 0.35, fontSize: 10.5, color: ACCENT, italic: true, fontFace: "Calibri", margin: 0 });
}

// ─── SLIDE 13 – SYNAPTIC VESICLE TYPES ────────────────────────────────────────
{
  const s = addSlide();
  header(s, "Synaptic Vesicle Types & NT Storage", "Predetermines what is released");

  const vTypes = [
    {
      name: "Small Clear Vesicles", size: "~50 nm", color: ACCENT,
      content: "Amino acids & amines (Glutamate, GABA, ACh, Glycine)",
      release: "Released in response to single or low-frequency action potentials",
      location: "Clustered at active zones"
    },
    {
      name: "Large Dense-Core Vesicles (LDCV)", size: "~100 nm", color: GOLD,
      content: "Peptide neurotransmitters (Substance P, Enkephalins, NPY, CRH)",
      release: "Require high-frequency burst stimulation for adequate Ca²⁺ levels",
      location: "Distributed throughout terminal, not restricted to active zones"
    },
    {
      name: "Co-release", size: "Mixed", color: "C77DFF",
      content: "Same terminal often contains both types – amine + peptide co-transmission",
      release: "Differential release based on firing pattern: low freq → amine; high freq → peptide",
      location: "Frequency-dependent modulation of postsynaptic response"
    }
  ];

  vTypes.forEach((v, i) => {
    const bx = i * 3.25 + 0.15;
    s.addShape(pres.ShapeType.rect, { x: bx, y: 1.0, w: 3.1, h: 4.35, fill: { color: "0A1E30" }, line: { color: v.color, pt: 2 } });
    s.addShape(pres.ShapeType.ellipse, { x: bx + 1.1, y: 1.1, w: 0.9, h: 0.9, fill: { color: v.color }, line: { type: "none" } });
    s.addText(v.size, { x: bx + 1.1, y: 1.1, w: 0.9, h: 0.9, fontSize: 9, bold: true, color: BG, align: "center", valign: "middle", fontFace: "Calibri", margin: 0 });
    s.addText(v.name, { x: bx + 0.1, y: 2.1, w: 2.9, h: 0.55, fontSize: 12, bold: true, color: v.color, align: "center", fontFace: "Calibri", margin: 0 });
    [["Content:", v.content], ["Release:", v.release], ["Location:", v.location]].forEach(([lbl, val], j) => {
      s.addText([
        { text: lbl + " ", options: { bold: true, color: GOLD } },
        { text: val, options: { color: LIGHT } }
      ], { x: bx + 0.1, y: 2.7 + j * 0.78, w: 2.9, h: 0.72, fontSize: 10.5, fontFace: "Calibri", valign: "top", margin: 0 });
    });
  });
}

// ─── SLIDE 14 – SYNAPTIC PLASTICITY ───────────────────────────────────────────
{
  const s = addSlide();
  header(s, "Synaptic Plasticity", "Activity-dependent modification of predestined connections");

  s.addText("Synaptic predestination establishes the direction of communication; plasticity modifies its strength.",
    { x: 0.3, y: 0.92, w: 9.4, h: 0.4, fontSize: 13, color: ACCENT, italic: true, fontFace: "Calibri", margin: 0 });

  const plastitypes = [
    { type: "Short-term Facilitation", time: "ms–s", mech: "Residual Ca²⁺ in terminal → enhanced vesicle fusion on repeated stimulation", sig: "Boosts high-frequency signal transmission", col: ACCENT },
    { type: "Short-term Depression", time: "ms–s", mech: "Vesicle pool depletion at high-P synapses → reduced NT release per AP", sig: "Low-pass filter effect on repetitive inputs", col: ACCENT2 },
    { type: "Long-term Potentiation (LTP)", time: "hours–years", mech: "NMDA receptor activation → Ca²⁺ influx → AMPA receptor insertion, CaMKII phosphorylation", sig: "Molecular basis of learning and memory", col: GOLD },
    { type: "Long-term Depression (LTD)", time: "hours–years", mech: "Low-level Ca²⁺ rise → AMPA receptor internalisation (endocytosis)", sig: "Synaptic pruning, motor learning, forgetting", col: "C77DFF" },
    { type: "Homeostatic (Synaptic Scaling)", time: "days", mech: "Global up/down regulation of all synapses to maintain network stability", sig: "Prevents runaway excitation or silencing", col: "FF6B6B" },
  ];

  plastitypes.forEach((p, i) => {
    s.addShape(pres.ShapeType.rect, { x: 0.15, y: 1.38 + i * 0.81, w: 2.8, h: 0.72, fill: { color: p.col }, line: { type: "none" } });
    s.addText([{ text: p.type, options: { bold: true, color: BG, fontSize: 11 } }, { text: "\n" + p.time, options: { color: BG, fontSize: 9, italic: true } }],
      { x: 0.15, y: 1.38 + i * 0.81, w: 2.8, h: 0.72, align: "center", valign: "middle", fontFace: "Calibri", margin: 0 });
    s.addShape(pres.ShapeType.rect, { x: 3.05, y: 1.38 + i * 0.81, w: 3.6, h: 0.72, fill: { color: "0A1E30" }, line: { color: p.col, pt: 1 } });
    s.addText(p.mech, { x: 3.1, y: 1.38 + i * 0.81, w: 3.5, h: 0.72, fontSize: 10, color: LIGHT, valign: "middle", fontFace: "Calibri", margin: 0 });
    s.addShape(pres.ShapeType.rect, { x: 6.75, y: 1.38 + i * 0.81, w: 3.1, h: 0.72, fill: { color: "0A1E30" }, line: { color: p.col, pt: 1 } });
    s.addText(p.sig, { x: 6.8, y: 1.38 + i * 0.81, w: 3.0, h: 0.72, fontSize: 10, color: GOLD, italic: true, valign: "middle", fontFace: "Calibri", margin: 0 });
  });

  // column headers
  ["Type  |  Timescale", "Mechanism", "Significance"].forEach((h, i) => {
    const xArr = [0.15, 3.05, 6.75]; const wArr = [2.8, 3.6, 3.1];
    s.addText(h, { x: xArr[i], y: 1.2, w: wArr[i], h: 0.32, fontSize: 11, bold: true, color: ACCENT, align: "center", fontFace: "Calibri", margin: 0 });
  });
}

// ─── SLIDE 15 – SUMMARY & CLINICAL PEARLS ─────────────────────────────────────
{
  const s = addSlide();
  header(s, "Summary & Clinical Pearls", "Key concepts for exams & clinical practice");

  // 6 summary boxes
  const summaries = [
    { txt: "Synapse = specialized junction; classified by structure & function", col: ACCENT },
    { txt: "Electrical (gap junction): fast, bidirectional, connexins; Chemical: NT-mediated, unidirectional, amplifiable", col: ACCENT2 },
    { txt: "Structural types: Axodendritic > Axosomatic > Axoaxonic > Axospinous > Dendrodendritic", col: GOLD },
    { txt: "Functional: Excitatory (EPSP, Glu/ACh) vs Inhibitory (IPSP, GABA/Glycine) — Gray type I vs II", col: "00C853" },
    { txt: "Predestination = one-way transmission because vesicles & active zones are presynaptic; receptors postsynaptic", col: "C77DFF" },
    { txt: "Plasticity (LTP/LTD) modifies synaptic strength — molecular basis of memory, learning, and drug action", col: "FF6B6B" },
  ];

  summaries.forEach((sm, i) => {
    const bx = (i % 2) * 4.9 + 0.15;
    const by = Math.floor(i / 2) * 1.45 + 1.0;
    s.addShape(pres.ShapeType.rect, { x: bx, y: by, w: 4.7, h: 1.3, fill: { color: "0A1E30" }, line: { color: sm.col, pt: 2 } });
    s.addShape(pres.ShapeType.rect, { x: bx, y: by, w: 0.25, h: 1.3, fill: { color: sm.col }, line: { type: "none" } });
    s.addText(sm.txt, { x: bx + 0.35, y: by + 0.1, w: 4.2, h: 1.1, fontSize: 11.5, color: LIGHT, valign: "middle", fontFace: "Calibri", margin: 0 });
  });

  // clinical pearls strip
  s.addShape(pres.ShapeType.rect, { x: 0, y: 5.2, w: 10, h: 0.425, fill: { color: ACCENT2 }, line: { type: "none" } });
  s.addText("Clinical Pearls: Myasthenia Gravis (ACh-R antibodies) | Botulinum toxin (SNARE cleavage) | Parkinson's (dopaminergic synapse loss) | Benzodiazepines (GABA-A agonists) | SSRIs (serotonin reuptake inhibition)",
    { x: 0.2, y: 5.2, w: 9.6, h: 0.425, fontSize: 9.5, color: "FFFFFF", align: "center", valign: "middle", fontFace: "Calibri", margin: 0 });
}

pres.writeFile({ fileName: "/home/daytona/workspace/synapse-pptx/Synapse_Classification_Predestination.pptx" })
  .then(() => console.log("DONE"))
  .catch(e => console.error(e));

cd /home/daytona/workspace/synapse-pptx && node build.js 2>&1

ls -lh /home/daytona/workspace/synapse-pptx/