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Blood collection tubes (vacutainer tubes) are color-coded by their cap color, and each color indicates a specific additive inside. Here is a complete list:
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blood collection vacutainer tube colors names additive complete list

Here is the complete list of blood collection (vacutainer) tube names by cap color:

Blood Collection Tube (Vacutainer) - Complete Color List

Cap ColorAdditiveCommon NameMain Uses
šŸ”“ RedNone (no additive)Plain Red / Clot TubeSerology, immunology, blood bank, crossmatch
🟔 Gold / SSTClot activator + gel separatorSerum Separator Tube (SST)Chemistry, LFTs, TFTs, serology
🟠 OrangeThrombinRapid Serum Tube (RST)Stat chemistry tests
šŸ”µ Light BlueSodium citrate (3.2%)Coagulation TubePT, INR, aPTT, coagulation studies
⚫ BlackSodium citrate (buffered)ESR TubeErythrocyte sedimentation rate (Westergren method)
🟢 GreenSodium heparin / Lithium heparinHeparin TubeAmmonia, lithium levels, stat chemistry
šŸ’š Light GreenLithium heparin + gelPlasma Separator Tube (PST)Chemistry panels (plasma)
🟣 Lavender / PurpleEDTA (K2 or K3)EDTA TubeCBC, blood film, HbA1c, blood group
🩷 PinkEDTA (K2)Blood Bank / Type & Screen TubeCrossmatch, transfusion medicine
🟤 Tan / BrownEDTA or HeparinLead TubeLead levels
šŸ”µ Royal BlueEDTA or no additive (metal-free)Trace Element TubeZinc, copper, lead, mercury, toxicology
⚪ GraySodium fluoride + Potassium oxalateFluoride/Oxalate TubeGlucose, lactate (prevents glycolysis)
🟔 YellowACD (Acid Citrate Dextrose)ACD TubeHLA typing, DNA studies, paternity testing
šŸ–¤ Yellow-BlackBroth mixtureBlood Culture BottleAerobic/anaerobic cultures, fungi
šŸ¤ Pearl / WhiteK2EDTA + gelMolecular TubePCR, molecular testing
šŸ”µ Dark BlueEDTA (trace metal-free)Toxicology TubeHeavy metal screening

Order of Draw (Standard)

  1. Blood culture bottles
  2. Royal Blue (no additive)
  3. Light Blue (sodium citrate)
  4. Red / Gold (SST)
  5. Green / Light Green (heparin)
  6. Lavender / Pink (EDTA)
  7. Gray (fluoride)
  8. Yellow (ACD)

Key memory tip: "Stop (Red) Before (Blue) Little (Light blue) Green (Green) Monkeys (Mauve/Lavender) Go (Gray) Yellow"
This order prevents additive carryover contamination between tubes during collection.
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