I have all the content needed. Now let me compile a comprehensive, slide-ready PPT content based entirely on Park's Textbook.
PPT Content: HIV Testing & Diagnosis
Source: Park's Textbook of Preventive and Social Medicine
SLIDE 1 - Title Slide
HIV Testing & Diagnosis
- HIV Testing Strategies
- ICTC
- HIV Counselling (Pre- & Post-test)
- Diagnostic Tests
- HIV Screening in Special Populations
Source: Park's Textbook of Preventive and Social Medicine
SLIDE 2 - Introduction
HIV Counselling & Testing Services - Overview
- The Basic Service Division of the Department of AIDS Control provides HIV counselling and testing services
- National programme offering these services since 1997
- Goal: Identify as many PLHIV as early as possible and link them to prevention, care and treatment
- Introduction of ART services in 2004 gave a major boost to these services
- Three main components:
- Integrated Counselling and Testing Centres (ICTC)
- Prevention of Parent-to-Child Transmission of HIV (PPTCT)
- HIV/Tuberculosis collaborative activities
SLIDE 3 - HIV Testing Strategies (WHO 5 C's)
WHO Principles for HIV Testing - The "5 C's"
| Principle | Meaning |
|---|
| Consent | Voluntary and informed |
| Confidentiality | Results not disclosed without permission |
| Counselling | Pre- and post-test counselling provided |
| Correct test results | Quality-assured testing |
| Connection | Linkage to care and treatment |
HIV testing is the gateway to prevention, treatment, care and support services.
SLIDE 4 - HIV Testing Strategies
Types of HIV Testing Approaches
- Client-Initiated Testing and Counselling (CITC): Person voluntarily seeks HIV testing
- Provider-Initiated Testing and Counselling (PITC): Medical provider advises testing to patients
- Community-based screening: Conducted by frontline health workers (ANMs) at sub-centre level
- Compulsory testing: WHO warns against this - leads to social rejection of HIV carriers and psychological consequences
- Diagnostic testing: Used to confirm suspected HIV infection, avoids over-testing
Two key models:
- Unlinked anonymous testing (for surveillance)
- Named testing with informed consent (for clinical care)
SLIDE 5 - ICTC (Integrated Counselling and Testing Centre)
What is ICTC?
A facility where a person is counselled and tested for HIV, either:
- Of their own free will (Client-initiated)
- As advised by a medical provider (Provider-initiated)
Functions of ICTC:
- Early detection of HIV
- Provision of basic information on modes of transmission and prevention
- Promoting behavioural change and reducing vulnerability
- Linking PLHIV with other HIV prevention, care and treatment services
Currently >15,000 ICTCs in India offering PPTCT services
SLIDE 6 - Types of ICTC
Classification of ICTC
A. Fixed Facility ICTCs
- Standalone ICTC (SA-ICTC)
- High client load
- Full-time counsellor + laboratory technician
- Located in medical colleges, district hospitals, sub-district hospitals, CHCs
- Facility-Integrated ICTC (F-ICTC)
- Set up below block levels at 24x7 PHCs
- Existing health facility staff trained in counselling & testing
- Also includes PPP-ICTCs in private facilities
B. Mobile ICTC
- Van with examination room, counselling space, blood collection facility
- Team: Health educator/ANM + Counsellor + Lab technician
- Serves hard-to-reach areas with flexible working hours
- Provides: HIV counselling & testing, syndromic management of STI/RTI, ANC, immunization
SLIDE 7 - Levels of HIV Counselling & Testing in India
Hierarchical System (Pyramid Model)
| Level | Facility |
|---|
| Community level | ANM-based community HIV screening |
| Sub-district / Block level | F-ICTC at 24x7 PHC |
| District level | SA-ICTC at District Hospitals/CHCs |
| State/Medical College level | SA-ICTC + Centres of Excellence |
SLIDE 8 - HIV Counselling (Pre-Test)
Pre-Test Counselling - Key Components
- Conducted BEFORE the HIV test
- Establishes rapport and ensures informed consent
- Provides information on:
- HIV transmission modes
- Meaning of test results (positive/negative/window period)
- Risk assessment of the client
- Benefits of knowing one's status
- Available care and support services
- Addresses fears, misconceptions and stigma
- Voluntary and confidential
SLIDE 9 - HIV Counselling (Post-Test)
Post-Test Counselling - Key Components
If Result is NEGATIVE:
- Explain window period (up to 3 months after exposure)
- Repeat test if recent high-risk exposure
- Reinforce risk reduction behaviour
- Discuss prevention strategies
If Result is POSITIVE:
- Emotional support and crisis counselling
- Disclosure support (partner, family)
- Referral to ART centre for treatment
- Information on PLHIV rights and confidentiality
- Counselling on nutrition, positive living, positive prevention
- Linkage to care and support centres
SLIDE 10 - Diagnostic Tests for HIV
Laboratory Tests and Their Significance
| Test | Type | Significance |
|---|
| HIV ELISA | Screening | Sensitivity >99.9%; repeatedly reactive results confirmed with Western blot |
| Western Blot | Confirmatory | Specificity >99.99% when combined with ELISA; gold standard |
| Rapid tests | Screening | Quick results; used in ICTCs |
| p24 antigen | Early detection | Indicates active HIV replication; positive before seroconversion |
| HIV Viral Load | Monitoring | Measures actively replicating virus; correlates with disease progression and ART response |
| CD4 count | Staging/Monitoring | Most widely used predictor; CD4 <200 cells/µL = high risk for AIDS-defining illness |
| CD4 percentage | Monitoring | CD4% <14% = high risk; may be more reliable than absolute count |
| B2-Microglobulin | Prognosis | Levels >3.5 mg/dL = rapid progression; not useful in IV drug users |
| CBC | General | Anaemia, neutropenia, thrombocytopenia common in advanced HIV |
SLIDE 11 - HIV Testing Strategy (NACP)
WHO/NACO Testing Strategies
- Strategy I: Single ELISA - for blood bank screening (high sensitivity required)
- Strategy II: Two sequential ELISAs - for surveillance
- Strategy III: Three sequential ELISAs with different antigens - for diagnosis in symptomatic patients
ICTC Testing Protocol:
- Rapid HIV tests used (three tests with different principles)
- Testing done on same day - results given on same day
- If all three tests positive = HIV Positive
- If discordant results = repeat testing / Western Blot confirmation
SLIDE 12 - HIV Screening in Special Populations
1. Pregnant Women (PPTCT Programme)
- Universal HIV testing offered to ALL pregnant women at ANC
- "Opt-out" approach - routine testing unless refused
- Started in India in 2002
- Goal: Eliminate mother-to-child transmission (MTCT)
- Lifelong ART (TDF+3TC+EFV) for all HIV+ pregnant women regardless of CD4 count
- ARV prophylaxis for infants from birth up to minimum 6 months
- Early Infant Diagnosis (EID) by HIV-DNA PCR at 6 weeks of age
2. TB Patients
- Joint NACP-RNTCP initiative since 2007-08
- HIV testing of all diagnosed TB patients (states with high HIV prevalence cover ~90%)
- Case fatality rate in HIV+TB = 13-14% vs <4% in HIV-negative TB
- HIV testing offered upstream (when TB symptoms present) since 2012
SLIDE 13 - HIV Screening in Special Populations (cont.)
3. High-Risk Groups (Sentinel Surveillance)
| Group | Category |
|---|
| IDU, MSM, FSW, Transgender | High risk groups |
| Single male migrants, Long distance truckers | Bridge population |
| Pregnant women at ANC | General population |
- Sample size: 250 (high-risk/bridge) / 400 (general)
- Frequency: Once in 2 years
- Testing strategy for high-risk: Unlinked anonymous with informed consent
- Blood specimen: Dried Blood Spot (DBS)
- Testing protocol: Two-test protocol
4. Blood Donors
- Mandatory HIV screening of all blood donations
SLIDE 14 - 90-90-90 Targets (UNAIDS)
India's Fast-Track Commitment to End AIDS by 2030
- 90% of all PLHIV know their HIV status
- 90% of all diagnosed PLHIV receive sustained ART
- 90% of all people on ART achieve viral suppression
"Test and Treat" policy: All HIV positive individuals, regardless of CD4 count, are eligible for ART
SLIDE 15 - Summary
Key Takeaways
- HIV testing is the entry point to HIV prevention and care cascade
- ICTC is the backbone of India's HIV testing infrastructure (>15,000 centres)
- WHO 5 C's govern all HIV testing services
- ELISA (screening) + Western Blot (confirmatory) remain diagnostic standards
- CD4 count and viral load are key monitoring tools
- Special populations - pregnant women, TB patients, and high-risk groups - require targeted testing strategies
- India follows "Test and Treat" policy - no CD4 threshold for ART initiation
All content sourced from: Park's Textbook of Preventive and Social Medicine (23rd Edition) - Community Medicine