50 mark university post graduate theory exam prepare point wise and no

50 mark university post graduate theory exam prepare point wise and not long paragraphs , Question : Classify advances in periodontal diagnostic testing. Describe in detail the advances in microbiological analysis.. Prepare answer using the uploaded references , provide long form of abbrevation if used

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Part I - Classification (~10 marks) Covers 9 categories with point-wise sub-items: gingival inflammation assessment, attachment loss assessment (5 generations of probes), radiographic advances, microbiological analysis, immunodiagnostics, molecular biology, biomarkers, genetic testing, and chairside tests.

Part II - Advances in Microbiological Analysis (~40 marks) - 14 sub-sections:

Introduction and rationale
Bacterial culturing and AST (Antimicrobial Susceptibility Testing)
Dark-field, phase contrast, and fluorescence microscopy
Chromatography and HPLC (High-Performance Liquid Chromatography)
Immunodiagnostic techniques - ELISA, RIA, flow cytometry, latex agglutination
Checkerboard DNA-DNA Hybridization and the 5 color complexes (Socransky 1998)
16S rRNA gene techniques and PCR variants (multiplex, nested, real-time, qPCR)
Open-ended 16S NGS (Next-Generation Sequencing) and SMDI (Subgingival Microbial Dysbiosis Index)
Shotgun metagenomics with ARG (Antibiotic Resistance Gene) detection
DNA sequencing and fingerprinting
Metatranscriptomics
Metaproteomics
GCF biomarkers table
Future directions

All abbreviations are expanded in a master list at the end. Every point is traceable to one of the 6 uploaded references.