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Ultrasound in Pregnancy — Comprehensive Guide
Obstetric ultrasound is the cornerstone of antenatal surveillance. It is used for dating, anatomy assessment, growth monitoring, fetal wellbeing, and Doppler evaluation across all trimesters.
Overview: Routine Scans in Pregnancy
| Scan | Gestational Age | Primary Purpose |
|---|
| Early viability scan | 6–8 weeks | Confirm intrauterine pregnancy, cardiac activity |
| Dating/nuchal scan | 11–13+6 weeks | Dating, NT measurement, aneuploidy screening |
| Anomaly scan | 18–20 weeks | Fetal anatomy, structural survey |
| Growth scan | 28, 32, 36 weeks | Growth velocity, EFW, liquor, presentation |
| Doppler studies | As indicated (>28 weeks) | Fetal wellbeing in high-risk pregnancies |
| Biophysical profile | As indicated (>28 weeks) | Acute/chronic fetal compromise assessment |
1. Early Viability Scan (6–8 Weeks)
Route: Transvaginal ultrasound (TVS) preferred for early pregnancy.
Key Findings:
| Finding | Detail |
|---|
| Gestational sac (GS) | First structure visible at ~4.5–5 weeks; mean sac diameter (MSD) measured |
| Yolk sac | Visible at ~5.5 weeks; confirms intrauterine pregnancy; normal size <6 mm |
| Fetal pole | Visible at ~6 weeks; CRL measured |
| Cardiac activity | Visible by 6–6.5 weeks; fetal heart rate (FHR) 100–160 bpm |
| Crown-rump length (CRL) | Gold standard for dating at this stage; accurate ±3–5 days |
Indications: Vaginal bleeding, pelvic pain, confirm IUP, exclude ectopic pregnancy, confirm viability.
Discriminatory zone: GS seen by TVS when β-hCG >1500–2000 IU/L; GS ≥25 mm without fetal pole = anembryonic pregnancy (blighted ovum).
2. Dating / Nuchal Translucency Scan (11–13+6 Weeks)
Per Management of Pregnancy (p. 79): "A first-trimester ultrasound to establish or confirm gestational age and estimated birth date and to confirm the presence of cardiac activity is advised."
Gestational Age Dating:
- CRL 45–84 mm — accurate to ±5 days
- If CRL >84 mm (>14 weeks), use head circumference (HC) for dating
- Supersedes LMP if discrepancy >7 days (first trimester) or >14 days (second trimester)
Nuchal Translucency (NT):
| Parameter | Normal | Abnormal |
|---|
| NT | <3.0 mm (<95th percentile for CRL) | ≥3.5 mm significantly increases risk |
| Nasal bone | Present (visible in >95% normal) | Absent in ~60–70% Down syndrome |
| Ductus venosus (DV) | Positive a-wave | Reversed a-wave → aneuploidy, cardiac defect |
| Tricuspid regurgitation | Absent | Present → increased T21 risk |
Combined First Trimester Screening:
- NT + maternal serum PAPP-A + free β-hCG + maternal age
- Detection rate: ~85–90% for T21 with 5% false positive rate
Additional First Trimester Findings:
- Number of fetuses, chorionicity (in twins: lambda vs. T-sign)
- Uterine anatomy (fibroids, septa)
- Ovarian pathology (corpus luteum cyst)
- Subchorionic hematoma
- Crown-rump length measurement
- Fetal heart rate assessment
3. Anomaly Scan (18–20 Weeks)
The most comprehensive structural survey of the fetus.
Standard mid-pregnancy diagnostic scan planes: facial profile (top), sagittal spine with "railway track" appearance (middle), and transcerebellar brain view (bottom)
A. Head and Brain
| Structure | What to Assess | Normal Findings |
|---|
| Skull shape | Integrity, shape | Oval/round; no defects |
| Cerebral ventricles | Lateral ventricle width | <10 mm (atrial width) |
| Cavum septum pellucidum (CSP) | Presence | Present; absent → holoprosencephaly, ACC |
| Choroid plexus | Echogenicity, shape | Fills ventricles; "butterfly" appearance |
| Cerebellum | Transverse diameter | Normal for GA; "banana sign" absent |
| Posterior fossa/cisterna magna | Depth | 2–10 mm; enlarged in Dandy-Walker |
| Thalami | Symmetry | Present and symmetric |
| Nuchal fold | Thickness (15–20 wks) | <6 mm; ≥6 mm → T21 risk |
Lemon sign: Frontal bone scalloping — seen with open neural tube defects (spina bifida).
Banana sign: Cerebellar obliteration — seen with spina bifida (Arnold-Chiari type II).
B. Face
| Structure | Findings |
|---|
| Profile | Micrognathia, frontal bossing |
| Lips | Cleft lip (best in coronal plane) |
| Nasal bone | Presence/absence |
| Orbits | Hypotelorism/hypertelorism; cyclopia |
| Palate | Hard palate integrity (limited by US) |
C. Neck
- Nuchal edema / cystic hygroma
- Neck masses (teratoma, goiter)
D. Spine
- Sagittal view: Continuity of vertebral bodies and posterior elements; "railway track" pattern
- Transverse view: Three ossification centres in each vertebra; intact skin covering
- Spina bifida: Open defect with absent/disrupted posterior elements
E. Thorax and Heart
Basic cardiac views (ISUOG minimum standard):
| View | Structures Assessed |
|---|
| 4-chamber view | LV, RV, LA, RA, AV valves, apex pointing left (levocardia), equal chamber sizes |
| LVOT (Left ventricular outflow tract) | Aorta arising from LV, ventricular septal integrity |
| RVOT (Right ventricular outflow tract) | Pulmonary artery arising from RV, crossing aorta |
| 3-vessel view (3VV) | PA, aorta, SVC in descending size left to right |
| 3-vessel tracheal view (3VT) | V-shaped confluence at ductal arch |
Heart normal parameters:
- Heart occupies ~1/3 of chest area
- Apex points left, 4-chamber axis ~45°
- No cardiomegaly (cardiac: thoracic ratio <0.5 by area)
- Normal rhythm; rate 120–160 bpm
Lungs:
- Homogeneous echogenicity
- Echogenic mass → CPAM (congenital pulmonary airway malformation) or sequestration
Diaphragm: Intact; bowel/stomach in abdomen, not chest (CDH: stomach/bowel in chest, mediastinal shift)
F. Abdomen
| Structure | Normal Findings |
|---|
| Stomach | Visible, fluid-filled, in left upper quadrant |
| Anterior abdominal wall | Intact; cord insertion normal |
| Liver | Homogeneous; intrahepatic vessels |
| Kidneys | Present bilaterally; echogenic cortex, hypoechoic medulla; pelvis <7 mm AP at 20 weeks |
| Bladder | Visible, filling/emptying cyclically |
| Bowel | Non-echogenic (echogenic bowel is a soft marker) |
| Umbilical cord | 3 vessels (2 arteries, 1 vein); 2-vessel cord is abnormal |
G. Limbs
- Long bones measured: femur length (FL), humerus length (HL)
- All four limbs, three segments each (proximal/mid/distal)
- Hands and feet: presence, position (clubfoot, rocker-bottom foot)
- Skeletal dysplasia: short, bowed, or fractured bones
H. Soft Markers for Aneuploidy
| Marker | Associated Aneuploidy |
|---|
| Choroid plexus cysts (CPCs) | Trisomy 18 |
| Echogenic intracardiac focus (EIF) | Trisomy 21 |
| Mild renal pelviectasis (RPE) | Trisomy 21 |
| Short femur/humerus | Trisomy 21 |
| Echogenic bowel | Trisomy 21, CF, CMV |
| Nuchal fold ≥6 mm | Trisomy 21 |
| Single umbilical artery | Trisomy 18, 13 |
I. Placenta and Liquor
- Placental site: anterior, posterior, fundal, lateral; low-lying if <2 cm from internal os
- Placental texture: homogeneous (Grade 0 at 18–20 weeks)
- Amniotic fluid index (AFI): 5–24 cm (normal); or deepest vertical pool (DVP) 2–8 cm
- Cervical length: measured transvaginally; <25 mm = short cervix, risk of PTB
4. Growth Scans (28, 32, 36 Weeks)
Repeated biometry to assess growth velocity and fetal wellbeing.
Biometric Parameters:
| Parameter | Abbreviation | Use |
|---|
| Biparietal diameter | BPD | Head size |
| Head circumference | HC | Best head parameter |
| Abdominal circumference | AC | Most sensitive for FGR; reflects liver glycogen stores |
| Femur length | FL | Skeletal growth |
| Estimated fetal weight | EFW | Hadlock formula (HC+AC+FL±BPD) |
Interpretation:
| Finding | Definition | Significance |
|---|
| SGA (small for GA) | EFW <10th centile | May indicate FGR |
| FGR | EFW <3rd centile OR <10th with Doppler changes | Placental insufficiency |
| LGA (large for GA) | EFW >90th centile | Macrosomia, GDM |
| Oligohydramnios | AFI <5 cm or DVP <2 cm | FGR, PPROM, post-dates |
| Polyhydramnios | AFI >24 cm or DVP >8 cm | GDM, fetal anomaly, idiopathic |
Placental Grading (Grannum):
| Grade | Timing | Findings |
|---|
| 0 | <28 weeks | Homogeneous, flat chorionic plate |
| I | 28–31 weeks | Subtle undulations, stippling |
| II | 32–35 weeks | Basal densities, indentations without through-transmission |
| III | >35 weeks | Complete cotyledon divisions, calcifications |
5. Doppler Studies
Used primarily in high-risk pregnancies (FGR, hypertension, diabetes, red cell isoimmunization).
Uterine Artery Doppler (11–13 weeks and 20–24 weeks):
- Normal: Low-resistance waveform; RI <0.58, PI <1.45
- Abnormal: High resistance, notching (bilateral > unilateral)
- Predicts pre-eclampsia and FGR
Umbilical Artery (UA) Doppler:
| Finding | Interpretation | Action |
|---|
| Normal S/D ratio (<3.0 at term) | Normal fetoplacental resistance | Routine monitoring |
| Elevated PI/RI | Early placental insufficiency | Increase surveillance |
| Absent end-diastolic flow (AEDF) | Severe FGR | Admit; deliver at 34 weeks |
| Reversed end-diastolic flow (REDF) | Critical FGR | Deliver promptly |
Middle Cerebral Artery (MCA) Doppler:
- Brain-sparing: MCA PI decreases (vasodilation) in response to hypoxia
- Cerebro-placental ratio (CPR) = MCA PI / UA PI; <1.0 indicates redistribution
- MCA PSV: Used to detect fetal anaemia (>1.5 MoM = significant anaemia)
Ductus Venosus (DV) Doppler:
- Reflects right heart filling pressure / venous pressure
- Absent or reversed a-wave = advanced cardiac compromise; imminent fetal death
- Triggers delivery decision in severe FGR
Sequence of Doppler Changes in FGR:
Elevated UA PI → AEDF → REDF
↓
Decreased MCA PI (brain-sparing)
↓
Decreased CPR
↓
Abnormal DV (absent/reversed a-wave)
↓
Abnormal CTG → Fetal death
6. Biophysical Profile (BPP)
The BPP is an ultrasound-based assessment of five fetal biophysical variables that reflect both acute (CNS-mediated) and chronic (placenta-mediated) fetal oxygenation status.
Developed by: Manning FA (1980).
Observation period: 30 minutes of real-time ultrasound.
The Five Components (Manning's BPP)
Each parameter scores 2 (present/normal) or 0 (absent/abnormal) — no score of 1.
| # | Parameter | Normal (Score = 2) | Abnormal (Score = 0) |
|---|
| 1 | Fetal Breathing Movements (FBM) | ≥1 episode of rhythmic breathing lasting ≥30 seconds in 30 min | Absent or <30 seconds total |
| 2 | Gross Body Movements | ≥3 discrete body/limb movements in 30 min | ≤2 movements |
| 3 | Fetal Tone | ≥1 episode of active extension with return to flexion (limb/trunk); opening/closing of hand | Absent extension-flexion; limbs in extension; no fetal movement |
| 4 | Amniotic Fluid Volume (AFV) | ≥1 pocket of AF measuring ≥2 cm in two perpendicular planes (DVP ≥2 cm) | Largest pocket <2 cm |
| 5 | Non-Stress Test (NST) | ≥2 accelerations of ≥15 bpm × ≥15 seconds in 20 minutes (reactive) | Non-reactive |
Maximum score: 10/10
Amniotic fluid is a chronic marker (reflects long-term renal perfusion and placental function).
FBM, movement, tone, NST are acute markers (reflect current CNS integrity).
Physiological Basis of BPP
| Variable | CNS Centre | Gestational Age Appears | Disappears First in Hypoxia |
|---|
| Fetal tone | Cortex/subcortex (earliest) | 7–8 weeks | Last to disappear |
| Body movements | Cortex (nuclei) | 9 weeks | 3rd to disappear |
| Fetal breathing | Ventral surface, 4th ventricle | 20–21 weeks | 2nd to disappear |
| NST (reactivity) | Posterior hypothalamus | 26–28 weeks | 1st to disappear |
| Amniotic fluid | Chronic — fetal renal function / placental perfusion | — | Chronic marker |
Order of disappearance with hypoxia (acute):
NST reactivity → Fetal breathing → Body movements → Fetal tone
This mirrors phylogenetic order (newer CNS functions lost first).
BPP Score Interpretation and Management
| Score | Interpretation | Perinatal Mortality Risk | Management |
|---|
| 10/10 | Normal, non-asphyxiated | 1/1000 | Routine care; repeat as indicated |
| 8/10 (normal fluid) | Normal | 1/1000 | Repeat in 1 week (or per protocol) |
| 8/10 (abnormal fluid) | Chronic compromise suspected | 89/1000 | Deliver if ≥36 weeks; consider delivery at <36 weeks |
| 6/10 | Equivocal — possible compromise | Variable | Repeat within 24 hours; deliver if mature or repeat abnormal |
| 4/10 | Probable fetal compromise | 91/1000 | Deliver (regardless of GA) |
| 2/10 | Definite fetal compromise | 125/1000 | Deliver immediately |
| 0/10 | Gross fetal compromise | 600/1000 | Deliver immediately |
Modified BPP (mBPP)
A simplified version used for routine surveillance:
- NST (acute marker) + AFI (chronic marker)
- Normal: Reactive NST + AFI ≥5 cm
- Abnormal: Non-reactive NST or AFI <5 cm → full BPP or delivery decision
- Sensitivity ~90% with less time required
Limitations of BPP
- False positives: fetal sleep cycles (20–40 min), maternal sedation, CNS depressants, prematurity (FBM less regular <26 weeks)
- False negatives: rare but possible in acute events
- Requires 30-minute observation window (time-consuming)
- Operator-dependent
BPP vs. NST vs. CST Comparison
| Test | What It Measures | Advantage | Limitation |
|---|
| NST | Fetal heart rate reactivity | Simple, non-invasive | High false-positive rate |
| CST (OCT) | FHR response to contractions | Sensitive for hypoxia | Invasive, CI in preterm |
| BPP | 5 variables (acute + chronic) | Comprehensive; low FPR | Time-consuming |
| Modified BPP | NST + AFI | Quick screening | Less comprehensive |
| Doppler | Blood flow resistance | Early FGR detection | Operator-dependent |
7. Special Ultrasound Assessments
Cervical Length (TVS)
- Measured transvaginally at 16–24 weeks
- Normal: >25 mm
- Short cervix: <25 mm → risk of preterm labour; consider cervical cerclage or progesterone
- Funnelling: Internal os dilatation; U-shaped or V-shaped
Placenta Praevia
- Low-lying placenta: lower edge <20 mm from internal os at term
- Major praevia: Placenta covers internal os
- TVS most accurate for assessment
- Anterior low-lying with previous CS → suspect placenta accreta spectrum
Multiple Pregnancy Ultrasound
| Chorionicity | Amnionicity | Ultrasound Marker | Risk |
|---|
| Dichorionic diamniotic | DCDA | Lambda (twin peak) sign | Lowest risk |
| Monochorionic diamniotic | MCDA | T-sign | TTTS, FGR |
| Monochorionic monoamniotic | MCMA | Single sac, cord entanglement | Highest risk |
Best determined at 11–13+6 weeks when lambda/T-sign is most reliable.
Summary: Key Ultrasound Parameters at a Glance
| Parameter | Normal Range | Significance if Abnormal |
|---|
| NT | <3.0 mm (11–13+6 wks) | Aneuploidy, cardiac defect |
| Lateral ventricle width | <10 mm | Ventriculomegaly |
| Cisterna magna | 2–10 mm | Dandy-Walker if enlarged |
| Nuchal fold (15–20 wks) | <6 mm | T21 |
| Cardiac: thoracic ratio | <0.5 | Cardiomegaly |
| Renal pelvis AP (20 wks) | <7 mm | Pelviectasis |
| UA S/D ratio (term) | <3.0 | Placental insufficiency |
| MCA PSV | <1.5 MoM | Fetal anaemia |
| AFI | 5–24 cm | Oligo/polyhydramnios |
| DVP | 2–8 cm | Oligo/polyhydramnios |
| Cervical length | >25 mm | Preterm birth risk |
| BPP | 8–10/10 | Normal |
| CPR | >1.0 | Redistribution if <1.0 |
The biophysical profile remains the most comprehensive single test for fetal wellbeing, integrating both acute CNS-mediated reflexes and chronic placental function into a structured scoring system. It is used in conjunction with Doppler studies in high-risk pregnancies for optimal surveillance.