1 What all important properties of light contribute in image formation of microscope? How refractive index affects the resolution of microscope? ( 2 What is the principle of imaging in a compound microscope? Draw a ray diagram to show the image formation in microscope. 3 What is the difference between Bright field and Dark field microscope? How does magnification of microscope relate to focal length.( 4 Elaborate about the principle of phase contrast microscope and its advantage over the compound microscope? 5 Elaborate about fluorescence with help of Jablonski diagram? Describe in details about fluorescence microscope ? 6 What is Normaski prism? Describe the working principle of Differential interference microscope? 7 What is the advantage of using pinholes in confocal microscope? Describe the working principle and application of confocal microscope. 8 Describe out enantiomers. Which instrument is used to determine the optical purity of a compound. 9 Describe the advantage of Electron microscope over light microscope. Elaborate about Scanning Electron Microscope principle and its application. 10 Elaborate about the instrumentation and principle of Transmission Electron Microscope.Mention its biological significance. (4+4)