MBBS 1st Year Biochemistry Practical – Important Topics Viva + Practic

MBBS 1st Year Biochemistry Practical – Important Topics (Viva + Practical Exam) 1. Urine Analysis (Most Important) Normal urine constituents Abnormal urine constituents Tests for: Protein (Albumin) Glucose (Benedict's test) Ketone bodies (Rothera's test) Bile salts (Hay's test) Bile pigments (Fouchet's test) 2. Instruments & Spotters Colorimeter Spectrophotometer pH meter Centrifuge Flame photometer Electrophoresis apparatus Chromatography apparatus 3. Principles Asked in Viva Beer-Lambert law Colorimetry Spectrophotometry Buffer and pH Chromatography Electrophoresis ELISA principle 4. Estimations Blood glucose (GOD-POD method) Serum bilirubin Blood urea Serum creatinine Total protein and A:G ratio Cholesterol Triglycerides SGOT (AST) and SGPT (ALT) Alkaline phosphatase (ALP) 5. Important Clinical Cases for Viva Diabetes mellitus Jaundice Renal failure Nephrotic syndrome Proteinuria Dyslipidemia Myocardial infarction Gout Liver disease 6. Frequently Asked Viva Questions Normal blood glucose value Normal urine pH Normal serum bilirubin Normal blood urea Normal serum creatinine Functions of liver and kidney tests Causes of glycosuria and proteinuria 7. Must-Read Spotting Topics Vitamins and deficiency diseases Amino acids Carbohydrates Enzyme inhibitors Tumor markers Chromatography patterns One-Day Revision Priority 1. Urine analysis 2. Colorimeter + Beer-Lambert law 3. Blood glucose estimation 4. LFT and KFT parameters 5. Clinical case discussions (Diabetes, Jaundice, Renal failure) 6. Spotters and instruments These topics cover the majority of questions commonly asked in first-year MBBS biochemistry

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MBBS 1st Year Biochemistry Practical – Important Topics (Viva + Practical Exam) 1. Urine Analysis (Most Important) Normal urine constituents Abnormal urine constituents Tests for: Protein (Albumin) Glucose (Benedict's test) Ketone bodies (Rothera's test) Bile salts (Hay's test) Bile pigments (Fouchet's test) topics cover the majority of questions commonly asked in first-year MBBS biochemistry practicals and viva examinations.

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1. PDF – Complete Study Notes

Urine Analysis Notes

PDF Document · PDF

6 Sections — Physical parameters, Normal constituents, Abnormal constituents, All 5 test procedures with step-by-step tables, Jaundice differential diagnosis, and 15 rapid-fire Viva Q&A pairs. Sourced directly from Harper's Biochemistry 32nd Ed. and Henry's Clinical Diagnosis.

2. PowerPoint Presentation

Urine Analysis Presentation

Presentation · PPTX

13 slides with dark navy + teal design — Title, Normal parameters, Constituents, Abnormal findings, one dedicated slide per test (Heat Coagulation, Benedict's, Rothera's, Hay's, Fouchet's), a colour-coded Benedict's chart, Summary table, Jaundice differential, Viva Q&A, and Exam Day Checklist.

3. Flashcard Q&A Sheet (Print & Cut)

Urine Analysis Flashcards

PDF Document · PDF

32 colour-coded flashcards in a 2-column print-and-cut format. Cards are grouped by category (Physical Parameters, Abnormal Constituents, each of the 5 tests, Jaundice/Clinical, Viva Key Facts) with Question on top and Answer below each pair.

Quick Revision Reminders

Key Fact	Remember
Rothera's misses what?	β-Hydroxybutyrate (most abundant in DKA at ~78%) - no keto group
Hay's positive = sulphur?	Sinks (bile salts lower surface tension)
Fouchet's positive colour?	Green (bilirubin → biliverdin)
Bile tests absent in which jaundice?	Haemolytic (unconjugated bilirubin is albumin-bound)
Microalbuminuria clinical significance?	Earliest sign of diabetic nephropathy (Harper's 32nd Ed.)
Benedict's specificity?	NOT specific - detects all reducing sugars