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Lab Report: Measurement of Blood Pressure Using a Sphygmomanometer
Course: Medical/Nursing Sciences
Date: July 27, 2026
Subject: Cardiovascular Physiology - Clinical Skills
1. Aim
To measure arterial blood pressure non-invasively using a mercury or aneroid sphygmomanometer by the auscultatory method, to identify Korotkoff sounds, to record systolic and diastolic pressures, and to classify the recorded readings according to established clinical guidelines.
2. Learning Objectives
- Understand the physiological basis of blood pressure measurement
- Correctly identify the five phases of Korotkoff sounds
- Accurately record systolic and diastolic blood pressure values
- Classify blood pressure readings using the ACC/AHA classification system
3. Background / Theory
Blood pressure (BP) is the lateral force exerted per unit area by blood against the arterial wall, expressed in millimetres of mercury (mmHg). It has two components:
- Systolic Blood Pressure (SBP): the peak pressure during ventricular contraction
- Diastolic Blood Pressure (DBP): the minimum pressure during ventricular relaxation
Blood pressure is the product of cardiac output and total peripheral resistance. It is regulated by cardiac function, blood volume, vascular tone, and the autonomic nervous system.
The Sphygmomanometer
The sphygmomanometer (from Greek: sphygmos = pulse; manos = thin/sparse; metron = measure) consists of:
- An inflatable cuff with an inner pneumatic bladder
- A rubber inflation bulb with a valve
- A manometer (mercury column or calibrated aneroid gauge) to read pressure in mmHg
- A release valve for controlled deflation
The device was invented by Scipione Riva-Rocci in 1896. Nikolai Korotkoff refined the technique in 1905 by introducing auscultation of arterial sounds, which are now named after him. - Tintinalli's Emergency Medicine, Comprehensive Study
The Auscultatory Method and Korotkoff Sounds
When the cuff is inflated above systolic pressure, the brachial artery is fully occluded and no blood flow occurs. As cuff pressure is slowly released, blood forces through the partially compressed vessel, producing turbulent flow - this turbulence creates vibrations in the arterial wall detected as sound through a stethoscope placed over the brachial artery at the antecubital fossa. - Guyton & Hall Textbook of Medical Physiology
The sounds heard during deflation are divided into five phases:
| Phase | Sound Character | Clinical Significance |
|---|
| Phase I | Sharp tapping / thumping | First sound heard = Systolic BP |
| Phase II | Blowing or swishing murmur | Turbulent flow as cuff partially occludes artery |
| Phase III | Louder, rhythmical thumping | Flow increasing through wider opening |
| Phase IV | Muffled, softer sounds | Cuff pressure approaching diastolic level |
| Phase V | Complete silence (disappearance) | Diastolic BP (clinically used in most cases) |
Figure: Sphygmomanometry - the five phases of Korotkoff sounds and their relationship to arterial pressure. - Medical Physiology (Boron & Boulpaep)
Note on diastolic reading: Most clinicians use Phase V (disappearance) as the diastolic pressure. However, in children, pregnant women, patients with aortic regurgitation, or after exercise, Phase IV (muffling) is used because sounds may persist even at very low pressures. - Ganong's Review of Medical Physiology, 26th Edition
The auscultatory method is accurate to within approximately 10% of direct intra-arterial catheter measurements. - Guyton & Hall Textbook of Medical Physiology
4. Apparatus / Materials
| Equipment | Specification |
|---|
| Sphygmomanometer | Mercury or aneroid type, calibrated |
| Stethoscope | Standard binaural, with diaphragm and bell |
| Blood pressure cuff | Standard adult (bladder width ~12-13 cm); or appropriate size |
| Subject | Adult, rested for at least 5 minutes |
| Recording chart | To document readings |
| Pen/pencil | For documentation |
Cuff sizing guideline (AHA recommendations): The pneumatic bladder should be 20% wider than the arm diameter, encircle at least 80% of the arm circumference, and be centred over the brachial artery. An undersized cuff overestimates blood pressure; an oversized cuff underestimates it. - Medical Physiology (Boron & Boulpaep)
5. Procedure
Pre-measurement preparation
- Ask the subject to rest quietly for at least 5 minutes before measurement. Avoid exercise, caffeine, smoking, or talking during this period.
- The subject should be seated comfortably with the arm supported at heart level (mid-sternal level), feet flat on the floor, and back supported.
- Remove any tight clothing from the upper arm.
- Select the appropriate cuff size.
Palpatory method (to estimate systolic pressure first)
- Locate the radial pulse at the wrist. Wrap the cuff snugly around the upper arm, 2-3 cm above the antecubital fossa, with the bladder centred over the brachial artery.
- Inflate the cuff while palpating the radial pulse. Note the pressure at which the radial pulse disappears.
- Inflate a further 20-30 mmHg above this level.
- Slowly deflate (2-3 mmHg/second). Note the pressure at which the radial pulse returns - this estimates the systolic pressure and prevents missing an auscultatory gap.
Auscultatory method (main measurement)
- Place the stethoscope diaphragm (or bell) gently but firmly over the brachial artery at the antecubital fossa (do not tuck it under the cuff).
- Inflate the cuff to 20-30 mmHg above the estimated systolic pressure from the palpatory step.
- Slowly release the valve at approximately 2-3 mmHg per second or per heartbeat.
- Systolic BP: Note the pressure reading on the manometer when the first tapping sound (Phase I Korotkoff) is heard.
- Continue deflating. Listen through phases II and III.
- Diastolic BP: Note the pressure reading when the sounds become muffled (Phase IV) or completely disappear (Phase V) - record both if there is a discrepancy.
- Deflate the cuff completely. Wait at least 1-2 minutes before repeating.
- Take two readings and record the average. If values differ by >5 mmHg, take a third measurement.
- Repeat on the other arm at the first visit and record both.
Documentation
- Record as: SBP / DBP mmHg (e.g., 118/76 mmHg), noting which arm was used, subject's position, time, and any relevant conditions.
6. Observations / Results Table
| Parameter | Reading 1 | Reading 2 | Reading 3 (if needed) | Average |
|---|
| Systolic BP (mmHg) - Right arm | | | | |
| Diastolic BP (mmHg) - Right arm | | | | |
| Systolic BP (mmHg) - Left arm | | | | |
| Diastolic BP (mmHg) - Left arm | | | | |
| Heart rate (beats/min) | | | | |
| Subject position | Sitting / Supine | | | |
| Time of measurement | | | | |
| Phase IV diastolic (if applicable) | | | | |
| Phase V diastolic (if applicable) | | | | |
Sample result (to be replaced with actual readings):
- Right arm: 118/76 mmHg (average of two readings)
- Left arm: 116/74 mmHg
- Heart rate: 72 bpm
- Both readings within 10 mmHg of each other (acceptable)
7. BP Classification
Based on the ACC/AHA Blood Pressure Classification System for Adults (from Harrison's Principles of Internal Medicine, 22nd Edition, 2025):
| Category | Systolic BP (mmHg) | | Diastolic BP (mmHg) |
|---|
| Normal | < 120 | and | < 80 |
| Elevated | 120-129 | and | < 80 |
| Stage 1 Hypertension | 130-139 | or | 80-89 |
| Stage 2 Hypertension | ≥ 140 | or | ≥ 90 |
Classification of sample result (118/76 mmHg): Normal blood pressure.
8. Discussion
Physiological significance of the readings
Systolic blood pressure reflects the peak force generated by left ventricular contraction. Diastolic blood pressure represents the elastic recoil of the arterial walls during ventricular relaxation and the resistance of the peripheral vasculature. Pulse pressure (SBP - DBP) reflects stroke volume and arterial compliance; normally 40-60 mmHg. Mean arterial pressure (MAP = DBP + 1/3 pulse pressure) approximates organ perfusion pressure.
Sources of error in sphygmomanometry
| Source of Error | Effect on Reading | How to Avoid |
|---|
| Cuff too small | Overestimates BP | Use correct cuff size |
| Cuff too loose | Overestimates BP | Apply snugly |
| Arm above heart level | Underestimates BP | Support at heart level |
| Arm below heart level | Overestimates BP | Elevate appropriately |
| Deflating too fast (>2-3 mmHg/sec) | Underestimates systolic, overestimates diastolic | Slow, steady deflation |
| Auscultatory gap not detected | Underestimates systolic | Use palpatory method first |
| Observer bias (digit preference) | Rounds to nearest 0 or 5 | Use automated device or blind recording |
| "White coat hypertension" | Overestimates true resting BP | Automated or home measurement |
| Recent exercise, caffeine, or stress | Overestimates resting BP | Ensure 5-minute rest |
The auscultatory gap
An auscultatory gap is a period of silence between Phase I and Phase II sounds, most commonly seen in elderly patients with arteriosclerosis. If the cuff is not inflated above the true systolic pressure, the examiner may record a falsely low systolic value. Performing the palpatory method first prevents this error.
Clinical relevance
The
ACC/AHA 2017 guidelines classify normal adult BP as below 120/80 mmHg. Hypertension is a major risk factor for stroke, myocardial infarction, heart failure, renal failure, and peripheral artery disease. A sustained difference of >10 mmHg between arms may indicate subclavian artery stenosis or aortic coarctation and warrants further investigation. -
Harrison's Principles of Internal Medicine, 22nd Edition
9. Precautions
- Ensure the manometer is at eye level and calibrated to zero before use.
- Never inflate the cuff beyond 200-220 mmHg unnecessarily; prolonged occlusion causes discomfort and petechiae.
- Allow a minimum 1-2 minute rest between repeated measurements on the same arm.
- Do not measure on an arm with an arteriovenous fistula, lymphedema, axillary node dissection, or IV line.
- Ensure the stethoscope is not pressed under the cuff (produces artefact sounds).
- Mercury sphygmomanometers must be stored upright and handled carefully to prevent mercury spillage (environmental hazard).
- Inform the patient about the procedure before starting.
10. Conclusion
Blood pressure was successfully measured using the sphygmomanometer and auscultatory method. The five phases of Korotkoff sounds were identified, with Phase I marking the systolic pressure and Phase V (or Phase IV in special populations) marking the diastolic pressure. The readings were classified according to the ACC/AHA classification system. Accurate blood pressure measurement is a fundamental clinical skill that depends on correct technique, appropriate equipment sizing, and patient preparation. The auscultatory method gives values within 10% of direct intra-arterial measurements and remains the gold standard for clinical BP assessment.
11. References
- Guyton AC, Hall JE. Guyton and Hall Textbook of Medical Physiology. Elsevier; 2021. Chapter 15: Clinical Methods for Measuring Systolic and Diastolic Pressures.
- Boron WF, Boulpaep EL. Medical Physiology. Elsevier; 2017. Chapter 17: Blood Pressure Measurement by Sphygmomanometry.
- Barrett KE, Barman SM, Brooks HL, Yuan JX. Ganong's Review of Medical Physiology, 26th ed. McGraw-Hill; 2019. Chapter 31: Auscultatory Method.
- Loscalzo J et al. Harrison's Principles of Internal Medicine, 22nd ed. McGraw-Hill; 2025. Chapter 288: Blood Pressure Measurement.
- Tintinalli JE et al. Tintinalli's Emergency Medicine: A Comprehensive Study Guide. McGraw-Hill. Sphygmomanometry.
- Cheung AK et al. International consensus on standardized clinic blood pressure measurement: A call to action. Am J Med. 2023;136:438.
Note on the diagram above: The figure from Medical Physiology (Boron & Boulpaep) illustrates the complete sphygmomanometry process - the left side shows cuff placement and stethoscope position over the brachial artery, while the right graphs show the five phases of Korotkoff sounds plotted against cuff pressure (red line) declining from above systolic to below diastolic. Phase I (tapping) corresponds to systolic; Phase IV (muffling) corresponds to diastolic.