No. Yeasts are fungi, so they are not truly classified as Gram-positive or Gram-negative like bacteria.

However, many yeasts appear Gram-positive (purple) on a Gram stain because their thick cell wall retains crystal violet. For example, Candida and Malassezia commonly appear as purple, oval budding yeast cells. Malassezia has been described as gram-positive on Gram stain in laboratory references.

Gram staining can help detect yeast in a specimen, but fungal stains such as KOH preparation, calcofluor white, PAS, or GMS are often more useful for confirmation and morphology.